Drought effects on the early development stages of Panicum virgatum L.: Cultivar differences

We studied the response to moderate drought and re-watering in 55-day-old plants of the lowland cv. Kanlow and the upland cv. Greenville. Plants were grown in a growth chamber; drought treatment was applied by water suppression until moderate stress was reached. The optimal harvest time was determin...

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Bibliographic Details
Published in:	Biomass & bioenergy Vol. 66; pp. 49 - 59
Main Authors:	Aimar, D., Calafat, M., Andrade, A.M., Carassay, L., Bouteau, F., Abdala, G., Molas, M.L.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01-07-2014 Elsevier
Subjects:	Agronomy. Soil science and plant productions Antioxidant enzymes Biological and medical sciences Drought Economic plant physiology Fundamental and applied biological sciences. Psychology General agronomy. Plant production Generalities Generalities. Agricultural and farming systems. Agricultural development Generalities. Production, biomass, yield. Quality Growth and development Life Sciences Panicum virgatum Stress hormones Stress marker genes Drought Panicum virgatum Stress marker genes Antioxidant enzymes Stress hormones Monocotyledones Genetic marker Early stage Superoxide dismutase Recovery Water stress Energy crop Enzymatic activity Catalase Gramineae Sesquiterpenes Angiospermae Abscisic acid Plant growth substance Developmental stage Salicylates Hormone Salicylic acid Terpenoid Enzyme Antioxidant Peroxidases Monoterpene Herbaceous plant Spermatophyta Oxidoreductases Fodder crop Phytohormone Cultivar
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Summary:	We studied the response to moderate drought and re-watering in 55-day-old plants of the lowland cv. Kanlow and the upland cv. Greenville. Plants were grown in a growth chamber; drought treatment was applied by water suppression until moderate stress was reached. The optimal harvest time was determined when the relative water content (RWC) in plants reached an average of 60%. The effects of drought and recovering on growth (i.e. plant height and leaf number), antioxidant enzymes (SOD and CAT), abscisic, salicylic and jasmonic acids levels (ABA, SA, JA), and water stress related genes were analyzed. Drought induced a decrease in growth in cv. Greenville but not in Kanlow. Drought tolerance in cv. Kanlow was correlated with higher antioxidant activities both in stress and recovery. Thus, we investigated stress hormones known to induce reactive oxygen species (ROS). ABA was the main hormone under stress, and increased higher in cv. Kanlow than in cv. Greenville. SA and JA basal levels were higher in well watered plants of cv. Kanlow. At gene levels, RAB18 and RD22 overexpressed after 24 h of re-watering in cv. Kanlow, resembling a “stress memory” mechanism described in other species. Further molecular markers of hormone effects (PR2) or drought (DREB2) well known in dicots seem not to be specific for Panicum virgatum. Overall, we found that cultivar Kanlow has an effective machinery of stress response, which makes it promising to be grown successfully in semi-arid regions. •We studied the response of P. virgatum to drought and recovery in two cultivars.•Cv. Kanlow showed high basal levels of SA and JA in well-watered plants.•Water stress triggered a major increase in ABA and antioxidant activity in cv Kanlow.•RAB 18 and RD22 were overexpressed under stress and after 24 h of recovery in cv Kanlow.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0961-9534 1873-2909
DOI:	10.1016/j.biombioe.2014.03.004