Evaluation of ImageJ software in ultrasonic image analysis: Follicular and luteal morphological characteristics of cattle

Evaluation of ImageJ software in ultrasonic image analysis: Follicular and luteal morphological characteristics of cattle

This study was conducted to compare the effectiveness of two methodologies in evaluating B- and Doppler-mode ultrasonic images: analysis using ultrasonic software and utilizing a computer with ImageJ software. To determine if ImageJ software utilization is an efficacious alternative to ultrasonic so...

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Published in:Animal reproduction science Vol. 236; p. 106907
Main Authors: Brito, Lindomar Sousa, da Silva Cavalcante, Ana Karina, Rodrigues, Alexandra Soares, Ferraz, Priscila Assis, Bittencourt, Rodrigo Freitas, Maggitti Junior, Luiz Di Paolo, Vasconcelos, Isabela Costa, Carôso, Bia Santos Souza, Lents, Maicon Pereira, Loiola, Marcus Vinicius Galvão, Madrigal-Valverde, Mónica, Bastos, Morgana Cardoso Brasileiro Borges, de Brito, Osvaldo Santos, de Lisboa Ribeiro Filho, Antônio
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-01-2022
Subjects:
Animals
Bioimage
Cattle
Corpus Luteum - cytology
Corpus Luteum - diagnostic imaging
Crossbred bovine
Doppler
Female
Image processing
Image Processing, Computer-Assisted - statistics & numerical data
Ovarian Follicle - cytology
Ovarian Follicle - diagnostic imaging
Reproduction
Software
Ultrasonics - instrumentation
Ultrasonics - methods
Crossbred bovine
Reproduction
Bioimage
Image processing
Doppler
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Summary:This study was conducted to compare the effectiveness of two methodologies in evaluating B- and Doppler-mode ultrasonic images: analysis using ultrasonic software and utilizing a computer with ImageJ software. To determine if ImageJ software utilization is an efficacious alternative to ultrasonic software device- analysis, there were comparisons of values when using the two methods for morphological and vascular characteristics of follicular dynamics and luteal function in 18 crossbred cattle. From day 8 of an ovarian dynamics synchronization treatment regimen period until the time of ovulation (Day 10), B-mode and power-flow ultrasonic cineloops were obtained every 12 h to assess follicular diameter, wall area, and wall blood perfusion area. On Day 14 after ovulation, US cineloops of ovaries were obtained in B mode and power flow to evaluate various morphological and vascular characteristics of the corpus luteum (CL), including luteal diameter, luteal area, and CL blood perfusion area. Cineloops were evaluated and analyzed using ultrasonic software, and in a computer with ImageJ software. To evaluate consistency in results between the two methods, there was evaluation utilizing paired t-test, Pearson correlation coefficient, Bland-Altman plot, and Linear Regression Test procedures to calculate proportion of bias between values for measurements of variables evaluated. Results indicated none of the values for variables before and after ovulation differed (P > 0.05). It, therefore, was concluded that utilization of ImageJ software is an efficacious biomedical technique to analyze ultrasonic images of morphological and vascular characteristics before and after ovulation in cattle. •This is the first study that evaluate the effectiveness of ImageJ software the analysis of ovarian structures blood flow.•Two effective alternatives for processing ultrasound images the follicular wall and CL in bovine females.•Bovine follicular and luteal structures images can be analyzed by ImageJ open source software.•ImageJ is suitable for the analysis of ultrasound images in B-mode and Doppler.
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ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2021.106907