Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Neurolysin oligopeptidase (E.C.3.4.24.16; Nln), a member of the zinc metallopeptidase M3 family, was first identified in rat brain synaptic membranes hydrolyzing neurotensin at the Pro-Tyr peptide bond. The previous development of C57BL6/N mice with suppression of Nln gene expression (Nln-/-), demon...

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 24; no. 20; p. 15190
Main Authors: Caprioli, Bruna, Eichler, Rosangela A. S, Silva, Renée N. O, Martucci, Luiz Felipe, Reckziegel, Patricia, Ferro, Emer S
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-10-2023
MDPI
Subjects:
adipose tissue
Amino acids
Animals
Body fat
Carbohydrates
Diet
diet-induced obesity
Energy
Females
Homeostasis
Insulin
intracellular peptides
Metabolism
neurolysin
Neuropeptides
neurotensin
Obesity
Peptides
Proteases
Proteins
Signal transduction
Type 2 diabetes
Brazil
Massachusetts
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Summary:Neurolysin oligopeptidase (E.C.3.4.24.16; Nln), a member of the zinc metallopeptidase M3 family, was first identified in rat brain synaptic membranes hydrolyzing neurotensin at the Pro-Tyr peptide bond. The previous development of C57BL6/N mice with suppression of Nln gene expression (Nln-/-), demonstrated the biological relevance of this oligopeptidase for insulin signaling and glucose uptake. Here, several metabolic parameters were investigated in Nln-/- and wild-type C57BL6/N animals (WT; n = 5–8), male and female, fed either a standard (SD) or a hypercaloric diet (HD), for seven weeks. Higher food intake and body mass gain was observed for Nln-/- animals fed HD, compared to both male and female WT control animals fed HD. Leptin gene expression was higher in Nln-/- male and female animals fed HD, compared to WT controls. Both WT and Nln-/- females fed HD showed similar gene expression increase of dipeptidyl peptidase 4 (DPP4), a peptidase related to glucagon-like peptide-1 (GLP-1) metabolism. The present data suggest that Nln participates in the physiological mechanisms related to diet-induced obesity. Further studies will be necessary to better understand the molecular mechanism responsible for the higher body mass gain observed in Nln-/- animals fed HD.
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Renée N. O. Silva is now a postdoctoral fellowship at Lawrence D. Longo MD Center for Perinatal Biology, Loma Linda University, Loma Linda, CA 92350, USA.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242015190