Search Results - "Cappione, Amedeo"

Germinal center exclusion of autoreactive B cells is defective in human systemic lupus erythematosus by Cappione, 3rd, Amedeo, Anolik, Jennifer H, Pugh-Bernard, Aimee, Barnard, Jennifer, Dutcher, Paul, Silverman, Gregg, Sanz, Iñaki

“…Breach of B cell tolerance is central to the pathogenesis of systemic lupus erythematosus (SLE). However, how B cell tolerance is subverted in human SLE is…”
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Novel ELISA Protocol Links Pre-Existing SARS-CoV-2 Reactive Antibodies With Endemic Coronavirus Immunity and Age and Reveals Improved Serologic Identification of Acute COVID-19 via Multi-Parameter Detection by Yuen, Rachel R, Steiner, Dylan, Pihl, Riley M F, Chavez, Elizabeth, Olson, Alex, Smith, Erika L, Baird, Lillia A, Korkmaz, Filiz, Urick, Patricia, Sagar, Manish, Berrigan, Jacob L, Gummuluru, Suryaram, Corley, Ronald B, Quillen, Karen, Belkina, Anna C, Mostoslavsky, Gustavo, Rifkin, Ian R, Kataria, Yachana, Cappione, 3rd, Amedeo J, Gao, Wenda, Lin, Nina H, Bhadelia, Nahid, Snyder-Cappione, Jennifer E

“…The COVID-19 pandemic has drastically impacted work, economy, and way of life. Sensitive measurement of SARS-CoV-2 specific antibodies would provide new…”
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Stimulation of PBMC and Monocyte-Derived Macrophages via Toll-Like Receptor Activates Innate Immune Pathways in HIV-Infected Patients on Virally Suppressive Combination Antiretroviral Therapy by Merlini, Esther, Tincati, Camilla, Biasin, Mara, Saulle, Irma, Cazzaniga, Federico Angelo, d'Arminio Monforte, Antonella, Cappione, 3rd, Amedeo J, Snyder-Cappione, Jennifer, Clerici, Mario, Marchetti, Giulia Carla

“…In HIV-infected, combination antiretroviral therapy (cART)-treated patients, immune activation and microbial translocation persist and associate with…”
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A New Population of Cells Lacking Expression of CD27 Represents a Notable Component of the B Cell Memory Compartment in Systemic Lupus Erythematosus by Wei, Chungwen, Anolik, Jennifer, Cappione, Amedeo, Zheng, Bo, Pugh-Bernard, Aimee, Brooks, James, Lee, Eun-Hyung, Milner, Eric C. B, Sanz, Inaki

“…Human memory B cells comprise isotype-switched and nonswitched cells with both subsets displaying somatic hypermutation. In addition to somatic hypermutation,…”
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Functionally distinct subsets of human NK cells and monocyte/DC-like cells identified by coexpression of CD56, CD7, and CD4 by Milush, Jeffrey M., Long, Brian R., Snyder-Cappione, Jennifer E., Cappione, Amedeo J., York, Vanessa A., Ndhlovu, Lishomwa C., Lanier, Lewis L., Michaëlsson, Jakob, Nixon, Douglas F.

“…The lack of natural killer (NK) cell–specific markers, as well as the overlap among several common surface antigens and functional properties, has obscured the…”
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A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-α and MIP1-β, a lack of IL-17, and a Th1-bias in males by Snyder-Cappione, Jennifer E, Tincati, Camilla, Eccles-James, Ijeoma G, Cappione, Amedeo J, Ndhlovu, Lishomwa C, Koth, Laura L, Nixon, Douglas F

“…NKT cells contribute to the modulation of immune responses and are believed to be important in the pathogenesis of autoimmune and infectious diseases, as well…”
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Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples by Nadler, Timothy, Chernokalskaya, Elena, Capito, Florian, Lento, Joseph, Amara, Ryan, Gutierrez, Sara, Cappione, Amedeo, Utzat, Christopher, Strug, Ivona, Skudas, Romas

“…Biological samples present a range of complexities from homogeneous purified protein to multicomponent mixtures. Accurate qualification of such samples is…”
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Distinguishing Latent from Active Mycobacterium tuberculosis Infection Using Elispot Assays: Looking Beyond Interferon-gamma by Tincati, Camilla, Cappione Iii, Amedeo J, Snyder-Cappione, Jennifer E

“…Mycobacterium tuberculosis (MTB) is a global heath epidemic, its threat amplified by HIV infection and the emergence of multidrug-resistant tuberculosis…”
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Human innate B cells: a link between host defense and autoimmunity? by Milner, Eric C B, Anolik, Jennifer, Cappione, Amedeo, Sanz, Iñaki

“…B cells play a variety of immunoregulatory roles through their antigen-presentation ability and through cytokine and chemokine production. Innate immune…”
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Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance by Pugh-Bernard, A E, Silverman, G J, Cappione, A J, Villano, M E, Ryan, D H, Insel, R A, Sanz, I

“…The study of human B cell tolerance has been hampered by difficulties in identifying a sizable population of autoreactive B lymphocytes whose fate could be…”
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Simplified size-based immune cell monitoring using a hand-held particle counting device by Smith, Janet L, Cappione, Amedeo

“…Abstract Biological samples are often heterogeneous cell mixtures that differ by type and functional or responsive capacity. Beyond differences in protein…”
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Abstract 5083: A comprehensive workflow for screening and real-time analysis of cell invasion by Cappione, Amedeo J., Nadler, Timothy

“…Abstract Cell migration and invasion are crucial steps in many normal, as well as, aberrant physiological processes, in particular, the highly lethal…”
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CyTOF enables unprecedented resolution of intracellular readouts to reveal novel functional diversity of human T Cells by Mcconney, Christina, Smith-Mahoney, Erika, Cohen, Michael, Belkina, Anna, Selvanantham, Thirumahal, King, David, Loh, Christina, Cappione, Amedeo, Snyder-Cappione, Jennifer

“…Abstract Measuring functional signatures at the single-cell level in a cross-lineage manner provides key insights into several facets of immunology, including…”
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Lupus IgG VH4.34 Antibodies Bind to a 220-kDa Glycoform of CD45/B220 on the Surface of Human B Lymphocytes by Cappione, Amedeo J, Pugh-Bernard, Aimee E, Anolik, Jennifer H, Sanz, Inaki

“…Anti-lymphocyte autoantibodies are a well-recognized component of the autoimmune repertoire in human systemic lupus erythematosus (SLE) and have been…”
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Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays by Cappione, Amedeo, Smith, Janet, Mabuchi, Masaharu, Nadler, Timothy

“…Expression profiling on a large scale, as is the case in drug discovery, is often accomplished through use of sophisticated solid-phase protein microarrays or…”
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Abstract 877: Real-time imaging of adherent and non-adherent cell interactions: utility of an automated microfluidic trap platform to recapitulate in vivo cell culture microenvironment by Kannan, Rekha, Yeh, Victor, Helton, James, Cappione, Amedeo

“…Abstract The study of dynamic cell processes and their interactions is of crucial importance to understand the complexities of tissue microenvironments…”
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Dynamic characterization of the phagocytic process utilizing an automated microfluidic platform by Chen, Cindy, Yeh, Victor, Booth, Ross, Helton, James, cappione, amedeo

“…Abstract Phagocytosis is a complex pathway that involves removal of pathogens, foreign particles, or cell debris through engulfment by unique cell types. Such…”
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Rituximab improves peripheral B cell abnormalities in human systemic lupus erythematosus by Anolik, Jennifer H., Barnard, Jennifer, Cappione, Amedeo, Pugh‐Bernard, Aimee E., Felgar, Raymond E., Looney, R. John, Sanz, Iñaki

“…Objective B lymphocyte depletion has recently emerged as a promising approach to the treatment of systemic lupus erythematosus (SLE). As part of a phase I/II…”
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Optimized small molecule antibody labeling efficiency through continuous flow centrifugal diafiltration by Cappione, Amedeo, Mabuchi, Masaharu, Briggs, David, Nadler, Timothy

“…Protein immuno-detection encompasses a broad range of analytical methodologies, including western blotting, flow cytometry, and microscope-based applications…”
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Optimized antibody labeling efficiency through continuous centrifugal diafiltration (TECH1P.838) by Cappione, Amedeo, Mabuchi, Masaharu, Briggs, David, Nadler, Timothy

“…Abstract Immuno-detection encompasses a range of analytical methodologies. These assays, which quantify and/or localize expression of protein(s) in complex…”
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