Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase

We have previously shown that Mu restores an active DNA replication at non-permissive temperature in E. coli K12 ligts7 strains. In this paper we describe two new types of phage mutants for the Mu lig functions. The Mu ligts mutants are conditional lethals defective for both integration and replicat...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 8; no. 23; pp. 5859 - 5873
Main Authors:	Paolozzi, L., Ghelardini, P., Liebart, J.C., Capozzoni, A., Marchelli, C.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 11-12-1980
Subjects:	Chromosome Deletion Chromosome Mapping DNA Ligases - genetics DNA Replication Escherichia coli Escherichia coli - genetics Lysogeny Models, Biological Mutation Polynucleotide Ligases - genetics RNA Phages - genetics Temperature Virus Replication
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Summary:	We have previously shown that Mu restores an active DNA replication at non-permissive temperature in E. coli K12 ligts7 strains. In this paper we describe two new types of phage mutants for the Mu lig functions. The Mu ligts mutants are conditional lethals defective for both integration and replication of DNA, unable to ‘complement’ the bacterial lig mutation; they map between B and lys. The mutants of the other type, on the other hand, are able to restore to a maximum level the activity impaired by the ligts7 mutation in the host. We suggest the hypothesis that the product of Mu lig gene could be part of a complex as a topoisomerase.
Bibliography:	istex:2DD42D18171B454BCC7FBB572EA9E560DBF06211 ArticleID:8.23.5859 ark:/67375/HXZ-6HB0MJF0-N ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/8.23.5859