Identification of NO/sGC signalling in the bulbospinal respiratory pathway after C2–C3 hemisection of the spinal cord in rats

Guanylyl cyclase (GC) as the effector molecule for nitric oxide (NO) plays a key role in the NO/cGMP signalling cascade. Based on these observations, our study focused on NO/sGC signalization in the bulbospinal respiratory pathway. The distribution of neuronal nitric oxide synthase (nNOS), β1 subuni...

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Published in:	Acta histochemica Vol. 113; no. 7; pp. 749 - 755
Main Authors:	Capková, Ľudmila, Dávidová, Alexandra, Pavel, Jaroslav, Kucharíková, Andrea, Radoňak, Jozef, Kuchárová, Karolína, Cigánková, Viera, Maršala, Martin, Lukáčová, Nadežda
Format:	Journal Article
Language:	English
Published:	Germany Elsevier GmbH 01-11-2011
Subjects:	Animals Bulbospinal respiratory pathway Cell survival Guanylate Cyclase - analysis Guanylate Cyclase - metabolism Male Neural Pathways - enzymology Neural Pathways - metabolism Nitric Oxide - analysis Nitric Oxide - metabolism Nitric oxide synthase Peripheral Nervous System Diseases - enzymology Peripheral Nervous System Diseases - metabolism Phrenic Nerve - enzymology Phrenic Nerve - metabolism Phrenic nucleus Rat Rats Rats, Wistar Signal Transduction Soluble guanylyl cyclase Spinal cord hemisection Spinal Cord Injuries - metabolism Spinal Cord Injuries - physiopathology Spinal Cord Injuries - surgery Ventral respiratory group FG NO CPP Rat Phrenic nucleus Soluble guanylyl cyclase β1sGC-IR cGMP SYN VRG Spinal cord hemisection rVRG sGC Ventral respiratory group Bulbospinal respiratory pathway NADPHd nNOS DRG SCI Nitric oxide synthase β1sGC GC NOS-IR PN
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Summary:	Guanylyl cyclase (GC) as the effector molecule for nitric oxide (NO) plays a key role in the NO/cGMP signalling cascade. Based on these observations, our study focused on NO/sGC signalization in the bulbospinal respiratory pathway. The distribution of neuronal nitric oxide synthase (nNOS), β1 subunit of soluble guanylyl cyclase (β1sGC) and synaptophysin (SYN) was explored in the upper part of the respiratory pathway after C2–C3 hemisection of the spinal cord in male Wistar rats. Unilateral injection of Fluorogold into the phrenic nucleus (PN) at C4 level and survival of animals for 2 days revealed many Fluorogold fluorescent neurons in the ventral respiratory group (VRG) of the medulla, mostly on the contralateral side. Under physiological conditions we noted nNOS-fluorescent terminals of VRG neurons around β1sGC fluorescent motoneurons in the PN. A strong depletion of nNOS/SYN fluorescent terminals was noted 8 days after hemisection around alpha motoneurons in the PN on the contralateral side. On the side of injury, nNOS/SYN fluorescent puncta were detected around phrenic motoneurons only sporadically. Phrenic alpha motoneurons responded to C2–C3 hemisection by a loss of β1sGC positivity. The results confirm, that β1sGC immunoreactive phrenic motoneurons are innervated by nNOS positive terminals coming from the VRG.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0065-1281 1618-0372
DOI:	10.1016/j.acthis.2010.11.003