Search Results - "Caperna, Thomas J"

Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins by Natarajan, Savithiry, Xu, Chenping, Caperna, Thomas J., Garrett, Wesley M.

“…Extraction of soybean seed proteins for two-dimensional polyacrylamide gel electrophoresis (2D–PAGE) and mass spectrometry analysis is challenging and…”
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Correction: Magnetic Cell Labeling of Primary and Stem Cell-Derived Pig Hepatocytes for MRI-Based Cell Tracking of Hepatocyte Transplantation by Roach, Dwayne R, Garrett, Wesley M, Welch, Glenn, Shuboni-Mulligan, Dorela D, Caperna, Thomas J, Talbot, Neil C, Shapiro, Erik M

“…[This corrects the article DOI: 10.1371/journal.pone.0123282.]…”
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Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis by Natarajan, Savithiry S, Xu, Chenping, Bae, Hanhong, Caperna, Thomas J, Garrett, Wesley M

“…A combined proteomic approach was applied for the separation, identification, and comparison of two major storage proteins, β-conglycinin and glycinin, in wild…”
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Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation by Roach, Dwayne R, Garrett, Wesley M, Welch, Glenn, Caperna, Thomas J, Talbot, Neil C, Shapiro, Erik M

“…Pig hepatocytes are an important investigational tool for optimizing hepatocyte transplantation schemes in both allogeneic and xenogeneic transplant scenarios…”
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Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells by Talbot, Neil C, Sparks, Wendy O, Powell, Anne M, Kahl, Stanislaw, Caperna, Thomas J

“…Feeder cells of irradiated mouse fibroblasts are commonly used for, and are generally necessary for, the in vitro maintenance and growth of many fastidious…”
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Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry by Xu, Chenping, Garrett, Wesley M., Sullivan, Joseph, Caperna, Thomas J., Natarajan, Savithiry

“…Soybean leaf proteins were separated by 2D gel electrophoresis and identified using MALDI-TOF-MS and LC-MS. To establish a proteomic reference map for soybean…”
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Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media by Caperna, Thomas J, Blomberg, Le Ann, Garrett, Wesley M, Talbot, Neil C

“…A serum-free, feeder cell-dependent, selective culture system for the long-term culture of porcine hepatocytes or cholangiocytes was developed. Liver cells…”
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Proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes by Natarajan, Savithiry, Xu, Chenping, Bae, Hanhong, Bailey, Bryan A., Cregan, Perry, Caperna, Thomas J., Garrett, Wesley M., Luthria, Devanand

“…We investigated proteomic and genomic profiles of glycinin, a family of major storage proteins in 16 different soybean genotypes consisting of four groups…”
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Proteome array identification of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses by Talbot, Neil C., Caperna, Thomas J.

“…Matrigel and similar commercial products are extracts of the Engelbreth-Holm–Swarm sarcoma that provide a basement-membrane-like attachment substrate or gel…”
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Proteomic analysis of the distribution of the major seed allergens in wild, landrace, ancestral, and modern soybean genotypes by Xu, Chenping, Caperna, Thomas J, Garrett, Wesley M, Cregan, Perry, Bae, Hanhong, Luthria, Devanand L, Natarajan, Savithiry

“…BACKGROUND: A comparative analysis of seed allergens from various soybean genotypes is crucial for identifying and eliminating potential allergens. We have…”
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Bovine Blastocyst-Derived Trophectoderm and Endoderm Cell Cultures: Interferon Tau and Transferrin Expression as Respective In Vitro Markers by Talbot, Neil C, Caperna, Thomas J, Edwards, J. Lannett, Garrett, Wes, Wells, Kevin D, Ealy, Alan D

“…Continuous cultures of bovine trophectoderm (CT-1 and CT-5) and bovine endoderm (CE-1 and CE-2) were initiated and maintained on STO feeder cells. CT-1 and…”
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Comparison of the Interferon-Tau Expression from Primary Trophectoderm Outgrowths Derived from IVP, NT, and Parthenogenote Bovine Blastocysts by Talbot, Neil C, Powell, Anne M, Ocon, Olga M, Caperna, Thomas J, Camp, Mary, Garrett, Wesley M, Ealy, Alan D

“…The expression of interferon-tau (IFN-) is essential for bovine embryo survival in the uterus. An evaluation of IFN- production from somatic cell nuclear…”
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Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors by Talbot, Neil C., Sparks, Wendy O., Phillips, Caitlin E., Ealy, Alan D., Powell, Anne M., Caperna, Thomas J., Garrett, Wesley M., Donovan, David M., Blomberg, Le Ann

“…Thirteen independent induced bovine trophectroderm (iBT) cell lines were established by reprogramming bovine fetal liver‐derived fibroblasts after viral‐vector…”
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Impact of solar Ultraviolet-B on the proteome in soybean lines differing in flavonoid contents by Xu, Chenping, Sullivan, Joe H., Garrett, Wesley M., Caperna, Thomas J., Natarajan, Savithiry

“…Two isolines of soybean Clark cultivar were grown in the field with or without natural levels of UV-B radiation. The proteins were extracted from…”
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Plasma clearance and tissue distribution of radiolabeled leptin in the chicken by McMurtry, John P, Ashwell, Christopher M, Brocht, Donna M, Caperna, Thomas J

“…Leptin is an adipose and liver tissue-derived secreted protein in chickens that has been implicated in the regulation of food intake and whole-body energy…”
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Establishment and characterization of feeder cell-dependent bovine fetal liver cell lines by Talbot, Neil C., Wang, Ling, Garrett, Wesley M., Caperna, Thomas J., Tang, Young

“…The establishment and initial characterization of bovine fetal liver cell lines are described. Bovine fetal hepatocytes were cultured from the liver of a 34-d…”
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A feeder-cell independent subpopulation of the PICM-19 pig liver stem cell line capable of long-term growth and extensive expansion by Talbot, Neil C., Caperna, Thomas J.

“…A method for the feeder-independent culture of PICM-19 pig liver stem cell line was recently devised, but the cell line’s growth was finite and the cells…”
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Isolation and Characterization of a Bovine Trophectoderm Cell Line Derived From a Parthenogenetic Blastocyst by Talbot, N.C, Caperna, T.J, Powell, A.M, Garrett, W.M, Ealy, A.D

“…A bovine trophectoderm cell line was established from a parthenogenetic in vitro-produced blastocyst. To initiate the cell line, 8-day parthenogenetic…”
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Identification of protein carbonyls in serum of the fetal and neonatal pig by Caperna, Thomas J., Shannon, Amy E., Blomberg, Le Ann, Garrett, Wesley M., Ramsay, Timothy G.

“…Oxidation of serum proteins leads to non-reversible carbonyl formation which alters their function and is associated with stress-related disease processes. The…”
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Identification of alpha-1 acid glycoprotein (AGP) as a potential marker of impaired growth in the newborn piglet by Caperna, Thomas J, Shannon, Amy E, Blomberg, Le Ann, Stoll, Margo, Ramsay, Timothy G

“…Two studies were conducted to investigate the relationship between circulating levels of haptoglobin and α-1 acid glycoprotein (AGP) and growth in neonatal…”
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