Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars

Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars

Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an a...

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Bibliographic Details
Published in:Crop breeding and applied biotechnology Vol. 22; no. 4
Main Authors: Azevedo, Ana Luisa Sousa, Souza, Flávia Rangel de, Campos, Rosiana Angélica, Reis, Daniele Ribeiro de Lima, Machado, Juarez Campolina, Machado, Marco Antonio, Lédo, Francisco José da Silva, Resende, Marcio
Format: Journal Article
Language:English
Published: Vicosa Crop Breeding and Applied Biotechnology 2022
Subjects:
AGRONOMY
Alleles
Animal nutrition
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Chemical fingerprinting
Cultivars
Deoxyribonucleic acid
DNA
DNA fingerprinting
Genetic fingerprinting
Grasses
Nucleotides
Panels
Polymorphism
Single-nucleotide polymorphism
Transcriptomes
elephant grass
breeding program
SSR
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Summary:Napier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification.
ISSN:1518-7853
1984-7033
1984-7033
DOI:10.1590/1984-70332022v22n4n39