Partial recovery of the damaged rat blood–brain barrier is mediated by adherens junction complexes, extracellular matrix remodeling and macrophage infiltration following focal astrocyte loss

Highlights • Paracellular VE-cadherin and β-catenin maintained in the absence of GFAP-astrocytes. • Demonstrates a different regulation mechanism for adherens and tight junction protein. • Extracellular matrix remodeling and inflammatory response following astrocyte loss. • Size-selective temporary...

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Published in:	Neuroscience Vol. 250; pp. 773 - 785
Main Authors:	Willis, C.L, Camire, R.B, Brule, S.A, Ray, D.E
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 10-10-2013 Elsevier
Subjects:	Adherens Junctions - physiology Animals Antigens, CD - biosynthesis Astrocytes - physiology basal lamina beta Catenin - biosynthesis Biological and medical sciences Blood-Brain Barrier - physiology Blotting, Western Cadherins - biosynthesis Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Claudin-5 - biosynthesis Electrophoresis, Polyacrylamide Gel Extracellular Matrix - physiology fibronectin Fibronectins - biosynthesis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Inferior Colliculi - cytology laminin Laminin - biosynthesis Macrophages - physiology Male Microscopy, Confocal Microscopy, Electron Neurology neurovascular unit Occludin - biosynthesis Rats Rats, Inbred F344 Sialic Acid Binding Ig-like Lectin 1 - biosynthesis tight junctions Tight Junctions - physiology VE-cadherin Vertebrates: nervous system and sense organs PBS PECAM-1 BBB phosphate-buffered saline Tris–Tween-buffered saline GFAP VE vascular endothelial blood–brain barrier fibronectin basal lamina TTBS platelet endothelial cell adhesion molecule-1 VE-cadherin laminin tight junctions GAPDH glial fibrillary acidic protein glyceraldehyde 3-phosphate dehydrogenase neurovascular unit Rat Neuroglia Rodentia Central nervous system Cell junction Astrocyte Cadherin Blood brain barrier Fibronectin Encephalon Vertebrata Mammalia Laminin Animal Tight junction Extracellular matrix Macrophage
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Summary:	Highlights • Paracellular VE-cadherin and β-catenin maintained in the absence of GFAP-astrocytes. • Demonstrates a different regulation mechanism for adherens and tight junction protein. • Extracellular matrix remodeling and inflammatory response following astrocyte loss. • Size-selective temporary barrier formed in the absence of tight junction proteins. • Represents a new approach to studying BBB integrity loss in demyelinating disorders.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0306-4522 1873-7544
DOI:	10.1016/j.neuroscience.2013.06.061