Relationships among isolates of Botrytis cinerea collected from greenhouses and field locations in Alberta, based on RAPD analysis

Relationships among isolates of Botrytis cinerea collected from greenhouses and field locations in Alberta, based on RAPD analysis

This study was undertaken to determine whether there was genetic diversity among isolates of Botrytis cinerea (teleomorph Botryotinia fuckeliana) collected from greenhouses and field locations in Alberta, Canada, and whether genetic diversity could be attributed to collection location and (or) host...

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Bibliographic Details
Published in:Canadian journal of plant pathology Vol. 28; no. 1; pp. 109 - 124
Main Authors: Calpas, J.T, Konschuh, M.N, Toews, C.C, Tewari, J.P
Format: Journal Article
Language:English
Published: Taylor & Francis Group 01-03-2006
Subjects:
Alberta
Botryotinia fuckeliana
Botrytis cinerea
crops
fields
fungal diseases of plants
genetic diversity
genetic variation
geographical variation
greenhouse production
greenhouses
host plants
Lycopersicon esculentum
microbial genetics
plant pathogenic fungi
random amplified polymorphic DNA analysis
random amplified polymorphic DNA technique
strain differences
strains
Alberta
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Summary:This study was undertaken to determine whether there was genetic diversity among isolates of Botrytis cinerea (teleomorph Botryotinia fuckeliana) collected from greenhouses and field locations in Alberta, Canada, and whether genetic diversity could be attributed to collection location and (or) host plant species. The study was conducted to provide basic information that is intended for use in developing effective greenhouse control strategies for B. cinerea. Ninety-one isolates of B. cinerea were collected from 20 host plant species in 31 greenhouse and field locations across the province. Relationships among subsets of the isolates were assessed, using random amplified polymorphic DNA (RAPD) analysis, and correlated with collection location and host plant. The first subset comprised 38 isolates collected from tomato (Lycopersicon esculentum) in eight greenhouses. The second subset was made up of 50 isolates from 10 host species collected in 10 greenhouses. The third subset consisted of 35 isolates from 17 host species from 23 greenhouses and 8 field locations. Random amplified polymorphic DNA analysis demonstrated distinct groups of B. cinerea within greenhouses in Alberta. Isolates in the three subsets clustered together based on the greenhouse of origin, but not on geographic region or host species. To our knowledge this is the first report of specialization in B. cinerea based on the greenhouse of origin.
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ISSN:0706-0661
1715-2992
DOI:10.1080/07060660609507277