Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method

Babesiosis is a tick borne disease (TBD) caused by parasites of the genus Babesia, with considerable worldwide economic, medical, and veterinary impact. Bovine babesiosis and other TBDs were considered responsible for 50% of the deaths of cattle that occurred in Mozambique in the first year after im...

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Published in:	Veterinary parasitology Vol. 153; no. 3; pp. 225 - 230
Main Authors:	Martins, Tiago M., Pedro, Olívia C., Caldeira, Rubina A., do Rosário, Virgílio E., Neves, Luís, Domingos, Ana
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 31-05-2008 Amsterdam; New York: Elsevier
Subjects:	analytical methods Animals Aspartic Acid Endopeptidases - genetics Aspartic protease B. bovis Babesia - enzymology Babesia - genetics Babesia - isolation & purification Babesia bigemina Babesia bovis Babesia bovis - enzymology Babesia bovis - genetics Babesia bovis - isolation & purification babesiosis Babesiosis - epidemiology Babesiosis - parasitology Babesiosis - veterinary Cattle cattle diseases Cattle Diseases - epidemiology Cattle Diseases - parasitology disease detection DNA DNA, Protozoan - chemistry DNA, Protozoan - genetics epidemiological studies Hot-start PCR Ixodidae Mozambique - epidemiology new methods polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Polymerase Chain Reaction - veterinary product evaluation Reproducibility of Results seminested hot-start polymerase chain reaction Sensitivity and Specificity Species Specificity Mozambique Babesia bigemina Aspartic protease Hot-start PCR B. bovis
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Summary:	Babesiosis is a tick borne disease (TBD) caused by parasites of the genus Babesia, with considerable worldwide economic, medical, and veterinary impact. Bovine babesiosis and other TBDs were considered responsible for 50% of the deaths of cattle that occurred in Mozambique in the first year after importation from neighbouring countries. Here, we present the detection of Babesia bigemina and Babesia bovis in cattle from Mozambique using two distinct PCR methods. For this study, blood samples were collected in one farm located near Maputo city. The DNA samples were analyzed using a previously described nested PCR and a novel hot-start PCR method. Primers were selected for the hot-start PCR based on the putative gene of an undescribed aspartic protease named babesipsin, present in both B. bovis and B. bigemina. The combination of hot-start polymerase and long primers (29–31 bp) were in this study determinant for the successful amplification and detection in only one PCR. With a seminested approach the sensitivity was further increased. The babesipsin seminested hot-start PCR was in this study more sensitive than the nested PCR. A total of 117 field samples were tested by seminested hot-start PCR, and 104 were positive for B. bigemina (90%), 97 were positive for B. bovis (82%), 86 were mixed infections (52%) and only 2 were negative for both Babesia species (1.7%). The results confirm that this area of Mozambique is endemic for babesiosis, and that this TBD should be regarded as a threat for imported cattle.
Bibliography:	http://dx.doi.org/10.1016/j.vetpar.2008.01.037 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-4017 1873-2550
DOI:	10.1016/j.vetpar.2008.01.037