Search Results - "Calcott, Mark J."

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution by Calcott, Mark J., Owen, Jeremy G., Ackerley, David F.

“…Non-ribosomal peptide synthetase (NRPS) enzymes form modular assembly-lines, wherein each module governs the incorporation of a specific monomer into a short…”
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High-Throughput Screening for Inhibitors of the SARS-CoV-2 Protease Using a FRET-Biosensor by Brown, Alistair S, Ackerley, David F, Calcott, Mark J

“…The global SARS-CoV-2 pandemic started late 2019 and currently continues unabated. The lag-time for developing vaccines means it is of paramount importance to…”
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Biosynthesis of novel Pyoverdines by domain substitution in a nonribosomal peptide synthetase of Pseudomonas aeruginosa by Calcott, Mark J, Owen, Jeremy G, Lamont, Iain L, Ackerley, David F

“…Pyoverdine is a fluorescent nonribosomal peptide siderophore made by fluorescent pseudomonads. The Pseudomonas aeruginosa nonribosomal peptide synthetase…”
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Generating Functional Recombinant NRPS Enzymes in the Laboratory Setting via Peptidyl Carrier Protein Engineering by Owen, Jeremy G, Calcott, Mark J, Robins, Katherine J, Ackerley, David F

“…Non-ribosomal peptide synthetases (NRPSs) are modular enzymatic assembly lines where substrates and intermediates undergo rounds of transformation catalyzed by…”
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Genetic manipulation of non-ribosomal peptide synthetases to generate novel bioactive peptide products by Calcott, Mark J., Ackerley, David F.

“…Non-ribosomal peptide synthetases (NRPS) are large modular enzymes that govern the synthesis of numerous biotechnologically relevant products. Their mode of…”
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Cheminformatics-Guided Exploration of Synthetic Marine Natural Product-Inspired Brominated Indole-3-Glyoxylamides and Their Potentials for Drug Discovery by Holland, Darren C, Prebble, Dale W, Calcott, Mark J, Schroder, Wayne A, Ferretti, Francesca, Lock, Aaron, Avery, Vicky M, Kiefel, Milton J, Carroll, Anthony R

“…Marine natural products (MNPs) continue to be tested primarily in cellular toxicity assays, both mammalian and microbial, despite most being inactive at…”
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The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes by Brown, Alistair S., Calcott, Mark J., Collins, Vincent M., Owen, Jeremy G., Ackerley, David F.

“…Objectives To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its…”
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A Genomic Survey of the Natural Product Biosynthetic Potential of Actinomycetes Isolated from New Zealand Lichens by Hou, Peng, Nowak, Vincent V, Taylor, Chanel J, Calcott, Mark J, Knight, Allison, Owen, Jeremy G

“…Actinomycetes are prolific producers of industrially valuable and medically important compounds. Historically, the most efficient method of obtaining compounds…”
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Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues by Hall, Kelsi R, Robins, Katherine J, Williams, Elsie M, Rich, Michelle H, Calcott, Mark J, Copp, Janine N, Little, Rory F, Schwörer, Ralf, Evans, Gary B, Patrick, Wayne M, Ackerley, David F

“…Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence…”
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Secondary metabolism in the lichen symbiosis by Calcott, Mark J, Ackerley, David F, Knight, Allison, Keyzers, Robert A, Owen, Jeremy G

“…Lichens, which are defined by a core symbiosis between a mycobiont (fungal partner) and a photobiont (photoautotrophic partner), are in fact complex…”
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Heterologous Expression of Epoxomicin in Escherichia coli by Messenger, Sarah R., Ackerley, David F., Calcott, Mark J.

“…Epoxomicin is an epoxyketone proteasome inhibitor with synthetic derivatives approved or under investigation for treatment of multiple myeloma. To leverage the…”
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Cyclotheonellazoles D‑I, Potent Elastase Inhibitory Thiazole-Containing Cyclic Peptides from Theonella sp. (2131) by Holland, Darren C., Schroder, Wayne A., Calcott, Mark J., Kaemmerer, Elke, Avery, Vicky M., Ekins, Merrick G.

“…Six new thiazole-containing cyclic peptides, the cyclotheonellazoles D–I (1–6), were isolated from the Australian marine sponge Theonella sp. (2131) with their…”
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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides by Messenger, Sarah R., McGuinniety, Edward M. R., Stevenson, Luke J., Owen, Jeremy G., Challis, Gregory L., Ackerley, David F., Calcott, Mark J.

“…The modular nature of nonribosomal peptide biosynthesis has driven efforts to generate peptide analogs by substituting amino acid-specifying domains within…”
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Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases by Calcott, Mark J, Ackerley, David F

“…Non-ribosomal peptide synthetase (NRPS) enzymes govern the assembly of amino acids and related monomers into peptide-like natural products. A key goal of the…”
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