Search Results - "Cai, Dawen"

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies by Tillberg, Paul W, Chen, Fei, Piatkevich, Kiryl D, Zhao, Yongxin, Yu, Chih-Chieh (Jay), English, Brian P, Gao, Linyi, Martorell, Anthony, Suk, Ho-Jun, Yoshida, Fumiaki, DeGennaro, Ellen M, Roossien, Douglas H, Gong, Guanyu, Seneviratne, Uthpala, Tannenbaum, Steven R, Desimone, Robert, Cai, Dawen, Boyden, Edward S

“…Improved expansion microscopy method preserves signal from fluorescent proteins and antibodies using off-the-shelf reagents. Expansion microscopy (ExM) enables…”
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Light microscopy based approach for mapping connectivity with molecular specificity by Shen, Fred Y., Harrington, Margaret M., Walker, Logan A., Cheng, Hon Pong Jimmy, Boyden, Edward S., Cai, Dawen

“…Mapping neuroanatomy is a foundational goal towards understanding brain function. Electron microscopy (EM) has been the gold standard for connectivity analysis…”
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A Weakly Supervised Multi-task Ranking Framework for Actor–Action Semantic Segmentation by Yan, Yan, Xu, Chenliang, Cai, Dawen, Corso, Jason J.

“…Modeling human behaviors and activity patterns has attracted significant research interest in recent years. In order to accurately model human behaviors, we…”
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Single molecule imaging reveals differences in microtubule track selection between Kinesin motors by Cai, Dawen, McEwen, Dyke P, Martens, Jeffery R, Meyhofer, Edgar, Verhey, Kristen J

“…Cells generate diverse microtubule populations by polymerization of a common alpha/beta-tubulin building block. How microtubule associated proteins translate…”
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Triple-Negative Breast Cancer Cells Recruit Neutrophils by Secreting TGF-β and CXCR2 Ligands by SenGupta, Shuvasree, Hein, Lauren E, Xu, Yang, Zhang, Jason, Konwerski, Jamie R, Li, Ye, Johnson, Craig, Cai, Dawen, Smith, Janet L, Parent, Carole A

“…Tumor associated neutrophils (TANs) are frequently detected in triple-negative breast cancer (TNBC). Recent studies also reveal the importance of neutrophils…”
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Ultra-small carbon fiber electrode recording site optimization and improved in vivo chronic recording yield by Welle, Elissa J, Patel, Paras R, Woods, Joshua E, Petrossians, Artin, Della Valle, Elena, Vega-Medina, Alexis, Richie, Julianna M, Cai, Dawen, Weiland, James D, Chestek, Cynthia A

“…Carbon fiber electrodes may enable better long-term brain implants, minimizing the tissue response commonly seen with silicon-based electrodes. The small…”
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Microtubule Acetylation Promotes Kinesin-1 Binding and Transport by Reed, Nathan A., Cai, Dawen, Blasius, T. Lynne, Jih, Gloria T., Meyhofer, Edgar, Gaertig, Jacek, Verhey, Kristen J.

“…Long-distance intracellular delivery is driven by kinesin and dynein motor proteins that ferry cargoes along microtubule tracks [1, 2]. Current models…”
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Weakly Supervised Actor-Action Segmentation via Robust Multi-task Ranking by Yan Yan, Chenliang Xu, Dawen Cai, Corso, Jason J.

“…Fine-grained activity understanding in videos has attracted considerable recent attention with a shift from action classification to detailed actor and action…”
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Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma by Adem, Bárbara, Bastos, Nuno, Ruivo, Carolina F., Sousa-Alves, Sara, Dias, Carolina, Vieira, Patrícia F., Batista, Inês A., Cavadas, Bruno, Saur, Dieter, Machado, José C., Cai, Dawen, Melo, Sonia A.

“…Pancreatic ductal adenocarcinoma (PDAC), a lethal disease, requires a grasp of its biology for effective therapies. Exosomes, implicated in cancer, are poorly…”
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A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection by Qian, Mengding, Cai, Dawen, Verhey, Kristen J, Tsai, Billy

“…The mechanisms by which receptors guide intracellular virus transport are poorly characterized. The murine polyomavirus (Py) binds to the lipid receptor…”
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The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis by Michki, Nigel S., Li, Ye, Sanjasaz, Kayvon, Zhao, Yimeng, Shen, Fred Y., Walker, Logan A., Cao, Wenjia, Lee, Cheng-Yu, Cai, Dawen

“…The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process…”
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Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mechanisms by Hammond, Jennetta W., Blasius, T. Lynne, Soppina, Virupakshi, Cai, Dawen, Verhey, Kristen J.

“…Long-distance transport in cells is driven by kinesin and dynein motors that move along microtubule tracks. These motors must be tightly regulated to ensure…”
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Two binding partners cooperate to activate the molecular motor Kinesin-1 by Blasius, T. Lynne, Cai, Dawen, Jih, Gloria T, Toret, Christopher P, Verhey, Kristen J

“…The regulation of molecular motors is an important cellular problem, as motility in the absence of cargo results in futile adenosine triphosphate hydrolysis…”
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A method for multiprotein assembly in cells reveals independent action of kinesins in complex by Norris, Stephen R, Soppina, Virupakshi, Dizaji, Aslan S, Schimert, Kristin I, Sept, David, Cai, Dawen, Sivaramakrishnan, Sivaraj, Verhey, Kristen J

“…Teams of processive molecular motors are critical for intracellular transport and organization, yet coordination between motors remains poorly understood…”
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Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition by Hammond, Jennetta W, Cai, Dawen, Blasius, T Lynne, Li, Zhe, Jiang, Yuyang, Jih, Gloria T, Meyhofer, Edgar, Verhey, Kristen J

“…Kinesin-3 motors drive the transport of synaptic vesicles and other membrane-bound organelles in neuronal cells. In the absence of cargo, kinesin motors are…”
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Identification of Neuronal Lineages in the Drosophila Peripheral Nervous System with a “Digital” Multi-spectral Lineage Tracing System by Veling, Macy W., Li, Ye, Veling, Mike T., Litts, Christopher, Michki, Nigel, Liu, Hao, Ye, Bing, Cai, Dawen

“…Elucidating cell lineages provides crucial understanding of development. Recently developed sequencing-based techniques enhance the scale of lineage tracing…”
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Kinesin-1 Structural Organization and Conformational Changes Revealed by FRET Stoichiometry in Live Cells by Cai, Dawen, Hoppe, Adam D., Swanson, Joel A., Verhey, Kristen J.

“…Kinesin motor proteins drive the transport of cellular cargoes along microtubule tracks. How motor protein activity is controlled in cells is unresolved, but…”
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Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains by Li, Ye, Walker, Logan A, Zhao, Yimeng, Edwards, Erica M, Michki, Nigel S, Cheng, Hon Pong Jimmy, Ghazzi, Marya, Chen, Tiffany Y, Chen, Maggie, Roossien, Douglas H, Cai, Dawen

“…Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among…”
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KIF5C S176 Phosphorylation Regulates Microtubule Binding and Transport Efficiency in Mammalian Neurons by Padzik, Artur, Deshpande, Prasannakumar, Hollos, Patrik, Franker, Mariella, Rannikko, Emmy H, Cai, Dawen, Prus, Piotr, Mågård, Mats, Westerlund, Nina, Verhey, Kristen J, James, Peter, Hoogenraad, Casper C, Coffey, Eleanor T

“…Increased phosphorylation of the KIF5 anterograde motor is associated with impaired axonal transport and neurodegeneration, but paradoxically also with normal…”
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Two-photon fluorescence resonance energy transfer stoichiometry in living cells by Bhagwat, Amar R., Flynn, Daniel, Brenner, Meredith H., Nunez, Marcos, Ogilvie, Jennifer P., Dawen Cai, Swanson, Joel A.

“…Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET)…”
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