Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates

The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of...

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Bibliographic Details
Published in:	Brazilian Journal of Pharmaceutical Sciences Vol. 54; no. 2
Main Authors:	Cavalcanti, Isabella Macário Ferro, Menezes, Talita Gomes Calaça, Campos, Luís André de Almeida, Ferraz, Milena Sales, Maciel, Maria Amélia Vieira, Caetano, Maria Nelly Psiotano, Santos-Magalhães, Nereide Stela
Format:	Journal Article
Language:	English
Published:	Sao Paulo Universidade de Sao Paulo Faculdade de Ciencias 01-01-2018 Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Universidade de São Paulo
Subjects:	Acids Antibiotics Antimicrobial agents Bacteria Drug delivery systems Drugs Laboratories Liposomes Methicillin-resistant Staphylococcus aureus (MRSA) Particle size PHARMACOLOGY & PHARMACY Staphylococcus infections Synergism Usnic acid Variance analysis β-lapachone Brazil Germany β-lapachone Usnic acid Liposomes Methicillin-resistant Staphylococcus aureus (MRSA) Synergism
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Summary:	The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction studies were carried out using the checkerboard method and epsilometer test (Etest). The interaction between VAN and β-lap or β-lap-lipo was synergistic (FICI = 0.453 and FICI = 0.358, respectively). An additive interaction between VAN and UA (FICI = 0.515) was found. UA-lipo resulted in synergism with VAN (FICI = 0.276). The Etest reproduced the results obtained by the checkerboard method for approximately 82% of the analysis. Thus, the present study demonstrated that VAN in combination with UA-lipo, β-lap or β-lap-lipo synergistically enhanced antibacterial activity against MRSA.
ISSN:	2175-9790 1984-8250 2175-9790
DOI:	10.1590/s2175-97902018000200203