Immune response to respiratory syncytial virus in young Brazilian children

We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 3.9 months) were tested...

Full description

Saved in:

Bibliographic Details
Published in:	Brazilian journal of medical and biological research Vol. 35; no. 10; pp. 1183 - 1193
Main Authors:	Queiróz, D A O, Durigon, E L, Botosso, V F, Ejzemberg, B, Vieira, S E, Mineo, J R, Yamashita, C, Hein, N, Lopes, C L, Cacharo, A L, Stewien, K E
Format:	Journal Article
Language:	English
Published:	Brazil Associação Brasileira de Divulgação Científica 01-10-2002
Subjects:	Age Factors Antibodies, Viral - immunology Antigens, Surface - immunology B-Lymphocytes - immunology Biomarkers Brazil Cytokines Cytokines - immunology Female Flow Cytometry Humans Immunity, Cellular Immunoenzyme Techniques Immunoglobulin G - immunology Infant Infant, Newborn Male Peripheral blood mononuclear cells Respiratory syncytial virus Respiratory Syncytial Virus Infections - immunology Respiratory Syncytial Virus, Human - immunology Surface antigens T-Lymphocytes - immunology Young children Brazil
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 +/- 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 +/- 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0100-879X 0100-879X 1414-431X
DOI:	10.1590/S0100-879X2002001000011