CYP1A2 is expressed along with CYP1A1 in the human lung

The expression and activity of CYP1A1 were examined in fresh, small-sized lung biopsy specimens from nine human subjects. CYP1A1 transcripts were detected by reverse transcription-polymerase chain reaction (RT-PCR) analysis of total lung RNA. CYP1A2 transcripts were detected in the RNA samples as we...

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Bibliographic Details
Published in:	Cancer letters Vol. 164; no. 1; pp. 25 - 32
Main Authors:	Wei, Cindy, Cacavale, Robert J., Kehoe, John J., Thomas, Paul E., Iba, Michael M.
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 10-03-2001 Elsevier
Subjects:	2-Amino-3,4-dimethylimidazo[4,5-f]quinoline Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - metabolism Benzo(a)pyrene - metabolism Benzo[ a]pyrene Biological and medical sciences Blotting, Western CYP1A1 CYP1A2 Cytochrome P-450 CYP1A1 - biosynthesis Cytochrome P-450 CYP1A1 - metabolism Cytochrome P-450 CYP1A2 - biosynthesis Cytochrome P-450 CYP1A2 - metabolism Dose-Response Relationship, Drug Enzymes and enzyme inhibitors Fluorenes - metabolism Fundamental and applied biological sciences. Psychology Human lung Humans Lung - metabolism Mice mRNA Mutagenicity Mutagenicity Tests Mutagens - metabolism Oxidoreductases Protein Quinolines - metabolism Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Human lung 2-Amino-3,4-dimethylimidazo[4,5-f]quinoline CYP1A2 CYP1A1 Benzo[ a]pyrene mRNA Mutagenicity Protein Surgical biopsy Human Enzyme Isozyme Gene product Mutagenicity testing Cytochrome P450 Lung Gene expression In vitro Tissue Ames test Metabolic activation Drug-metabolizing enzyme
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Summary:	The expression and activity of CYP1A1 were examined in fresh, small-sized lung biopsy specimens from nine human subjects. CYP1A1 transcripts were detected by reverse transcription-polymerase chain reaction (RT-PCR) analysis of total lung RNA. CYP1A2 transcripts were detected in the RNA samples as well, and bioactivation of 2-aminofluorene (2-AF) or 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), a CYP1A2-preferential activity, was catalyzed by the lung S 9 fractions also. Two major bands were detected in the whole homogenate by western blot analysis using CD3, a mouse anti rat CYP1A1 monoclonal that cross-reacts with rat CYP1A2 as well as with human CYP1A1 and CYP1A2. S 9 fractions from the tissues catalyzed the bioactivation of benzo[ a]pyrene (B[ a]P), a CYP1A1-preferential activity, to mutagens in the Ames assay. Our findings are in agreement with the known presence of CYP1A1 in the human lung, and provide strong evidence for the expression of catalytically functional CYP1A2 in the tissue.
ISSN:	0304-3835 1872-7980
DOI:	10.1016/S0304-3835(00)00712-6