Multifaceted and dual-edged native Trichoderma strains from subabul rhizospheric soil to combat Fusarium wilt disease - a sustainable approach

Multifaceted and dual-edged native Trichoderma strains from subabul rhizospheric soil to combat Fusarium wilt disease - a sustainable approach

Fusarium wilt and gummosis disease is a major concern in Subabul plantations, resulting in quantitative and qualitative wood losses to the paper and pulp industry. Growers currently rely on agrochemicals, which are not environmentally safe. Therefore, developing a sustainable control strategy employ...

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Bibliographic Details
Published in:Biocontrol science and technology Vol. 34; no. 9; pp. 843 - 857
Main Authors: Patil, Balanagouda, CT, Ganesh, Kotari, Pavitra, Rathinavelu, Rajkumar
Format: Journal Article
Language:English
Published: Abingdon Taylor & Francis 01-09-2024
Taylor & Francis Ltd
Subjects:
Agrochemicals
Biological control
Disease control
Effectiveness
Fungi
Fusarium
Gene sequencing
Inoculation
Metabolites
Multiplication
Native Trichoderma
Nutrients
Phylogeny
Plant diseases
Plant growth
Pulp & paper industry
Rooting
Siderophores
Soil investigations
subabul
Survival
sustainability
Trichoderma
Wilt
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Summary:Fusarium wilt and gummosis disease is a major concern in Subabul plantations, resulting in quantitative and qualitative wood losses to the paper and pulp industry. Growers currently rely on agrochemicals, which are not environmentally safe. Therefore, developing a sustainable control strategy employing biocontrol agents is necessary. For this, the investigation was performed to identify native Trichoderma strains from Subabul rhizospheric soil having antagonistic, disease control efficiency, and PGPR attributes. Six antagonistic Trichoderma (TR1 to TR6) strains were isolated and their efficacy was tested against Fusarium in-vitro by dual plate technique and volatile metabolites. Among these, Trichoderma strains TR1 and TR2 were found efficient in counteracting the growth of F. equiseti (70-90% inhibition) compared to control. The effectiveness was primarily attributed to the increased production of volatile metabolites, siderophores, and the ability to solubilise the nutrients (P and K). Further, six potent Trichoderma strains were identified as T. asperellum (TR1, TR2, TR3, TR6), and T. hamatum (TR4, TR5) through rDNA ITS gene sequencing and phylogenetic analysis. These isolates were mass-multiplied, formulated using Talc powder followed by bio-priming in rooting media. The application of Trichoderma into rooting media reduced the wilt disease caused by artificial inoculation of F. equiseti. Further, TR1 and TR2 were found promising with conferred protection against wilt, enhanced survival rate (10-15%), and improved growth compared to untreated control. Application of adapted TR1 and TR2 antagonists in rooting media during clonal multiplication has the potential wilt control efficiency, enhanced survival rate, and plant growth promotion in Subabul.
ISSN:0958-3157
1360-0478
DOI:10.1080/09583157.2024.2384945