PMN chemotactic factor produced by glass-adherent cells in the acute inflammation caused by Paracoccidioides brasiliensis

Intraperitoneal inoculation of BIO.A mice with P. brasiliensis induces an acute inflammatory infiltrate in which 40-50% of the cells are PMN leucocytes. Previous depletion of serotonin, prostaglandin, histamine and complement does not alter the course of inflammation. Complement-derived factors appe...

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Bibliographic Details
Published in:	British journal of experimental pathology Vol. 66; no. 1; pp. 57 - 65
Main Authors:	GARCIA CALICH, V. L, COPPI VAZ, C. A, BURGER, E
Format:	Journal Article
Language:	English
Published:	London Lewis 01-02-1985
Subjects:	Animals Ascitic Fluid - immunology Biological and medical sciences Cell Adhesion Chemotactic Factors - analysis Chemotactic Factors - biosynthesis Chemotaxis, Leukocyte Complement C5 - deficiency Experimental mycoses and models Female Infectious diseases Interleukin-8 Leukocyte Count Macrophages - immunology Macrophages - metabolism Medical sciences Mice Mice, Inbred Strains Molecular Weight Mycoses Neutrophils - immunology Paracoccidioidomycosis - immunology Paracoccidioidomycosis - metabolism Puromycin - pharmacology Cell culture Blastomycosis Mycosis Acute Blastomyces brasiliensis Granulocyte Glass Inflammation Adhesion Chemotactic factor Molecular weight Surface Fungi Infection Characterization Proteins Experimental disease Production Paracoccidioidomycosis Ascomycetes Inhibition Cell Thallophyta Peritoneum
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Summary:	Intraperitoneal inoculation of BIO.A mice with P. brasiliensis induces an acute inflammatory infiltrate in which 40-50% of the cells are PMN leucocytes. Previous depletion of serotonin, prostaglandin, histamine and complement does not alter the course of inflammation. Complement-derived factors appear to have no active participation in the process since C5-deficient mice depleted or not by Cobra venom factor (CoF) show the same kind of cellular influx. On the other hand, peritoneal cells incubated (6 h) with the fungus release a soluble factor that induces in vivo an active chemotaxis of PMN cells when inoculated i.p. The factor has the following characteristics: a) it is produced by adherent cells; b) it is protein in nature; c) its production is inhibited by incubation of peritoneal cells with 10 micrograms/ml puromycin and d) it has a molecular weight less than 15 000 daltons, as determined by gel filtration through a Sephadex G-75 column.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0007-1021