Human Epidermal Receptor-2 Expression in Prostate Cancer

Purpose: Efforts to conclusively establish that human epidermal receptor (HER)-2 overexpression is important to androgen-dependent carcinoma of the prostate (AD-CaP) or to progression to androgen independence (AI-CaP) have failed because of variability in tissue procurement, antibodies, immunostaini...

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Published in:	Clinical cancer research Vol. 9; no. 3; pp. 1087 - 1097
Main Authors:	CALVO, Benjamin F, LEVINE, Aaron M, MOHLER, James L, MARCOS, Mavie, COLLINS, Qu F, IACOCCA, Mary V, CASKEY, Laura S, GREGORY, Christopher W, YUHUA LIN, WHANG, Young E, EARP, H. Shelton
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Association for Cancer Research 01-03-2003
Subjects:	Androgens - metabolism Biological and medical sciences Carcinoma - metabolism Disease Progression Genes, erbB-2 - genetics Humans Immunohistochemistry In Situ Hybridization, Fluorescence Ligands Male Medical sciences Nephrology. Urinary tract diseases Polymerase Chain Reaction Prostatic Neoplasms - metabolism Receptor, ErbB-2 - biosynthesis Receptor, ErbB-2 - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Tumors of the urinary system Urinary tract. Prostate gland United States North America America Human Urinary system disease Prostate disease Gene overexpression Malignant tumor Gene expression Gene amplification Growth factor receptor Epidermal growth factor Caucasoid Male genital diseases Prostate African American
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Summary:	Purpose: Efforts to conclusively establish that human epidermal receptor (HER)-2 overexpression is important to androgen-dependent carcinoma of the prostate (AD-CaP) or to progression to androgen independence (AI-CaP) have failed because of variability in tissue procurement, antibodies, immunostaining procedures, and assessment methods. However, because some in vitro and animal model data correlate HER-2 overexpression with progression to androgen independence, trials of agents that target the HER-2 receptor are under way. To clarify human tumor findings, we studied HER-2 expression at the gene (DNA), mRNA, and protein levels in well-characterized CaP specimens. Experimental Design: Fifty AD-CaP and 25 AI-CaP specimens from similar numbers of Caucasian and African Americans were immunostained for HER-2 receptor. HER-2 mRNA levels were measured using real-time fluorescence quantitative PCR in patients for whom frozen specimens were available. HER-2 amplification was evaluated using fluorescent in situ hybridization. Results: HER-2 receptor immunostained in 52% of androgen-dependent and one (4%) androgen-independent tumor. HER-2 immunostaining was not related to age, race, serum prostate-specific antigen levels, or pathologic stage and Gleason grade. HER-2 overexpression was not detected in AI-CaP at the mRNA or gene level. Mean HER-2 mRNA expression was higher ( P < 0.05) in AD-CaP than AI-CaP (22,080 versus 15,496 HER-2 copies). HER-2 was not amplified in any of 20 AD-CaP or 19 AI-CaP specimens. Conclusions: HER-2 protein and message overexpression and HER-2 amplification were not found in AI-CaP.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1078-0432 1557-3265