Search Results - "COCKETT, M. I"

Matrix metalloproteinase degradation of elastin, type IV collagen and proteoglycan : a quantitative comparison of the activities of 95 kDa and 72 kDa gelatinases, stromelysins-1 and -2 and punctuated metalloproteinase (PUMP) by MURPHY, G, COCKETT, M. I, WARD, R. V, DOCHERTY, A. J. P

“…The abilities of the matrix metalloproteinases 95 kDa and 72 kDa gelatinases (type IV collagenases), stromelysins-1 and -2 and punctuated metalloproteinase…”
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The role of the C-terminal domain in collagenase and stromelysin specificity by MURPHY, G, ALLAN, J. A, WILLENBROCK, F, COCKETT, M. I, O'CONNEL, J. P, DOCHERTY, A. J. P

“…Recombinant human interstitial collagenase, an N-terminal truncated form, delta 243-450 collagenase, recombinant human stromelysin-1, and an N-terminal…”
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Distribution of heterotrimeric G-protein β and γ subunits in the rat brain by Betty, M, Harnish, S.W, Rhodes, K.J, Cockett, M.I

“…Heterotrimeric G-proteins, comprising α, β and γ subunits, have been shown to play a central role in coupling multiple receptors to a variety of enzymes and…”
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Assessment of the role of the fibronectin-like domain of gelatinase A by analysis of a deletion mutant by MURPHY, G, QUANG NGUYEN, COCKETT, M. I, ATKINSON, S. J, ALLAN, J. A, KNIGHT, C. G, WILLENBROCK, F, DOCHERTY, A. J. P

“…The properties of a deletion mutant delta V191-Q364 of gelatinase A, which represents the removal of the fibronectin-like type II repeats defined by exons 5-7,…”
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High Level Expression of Tissue Inhibitor of Metalloproteinases in Chinese Hamster Ovary Cells Using Glutamine Synthetase Gene Amplification by Cockett, M. I, Bebbington, C. R, Yarranton, G. T

“…We have used a glutamine synthetase (GS) gene as an amplifiable marker in Chinese hamster ovary (CHO) cells. GS was combined with an efficient transcription…”
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RGS7 Is Palmitoylated and Exists as Biochemically Distinct Forms by Rose, Jeremy J., Taylor, Jackie B., Shi, Jing, Cockett, Mark I., Jones, Philip G., Hepler, John R.

“…: Regulator of G protein signaling (RGS) proteins are GTPase‐activating proteins that modulate neurotransmitter and G protein signaling. RGS7 and its binding…”
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Antisense Oligonucleotide‐Induced Reduction in 5‐Hydroxytryptamine7 Receptors in the Rat Hypothalamus Without Alteration in Exploratory Behaviour or Neuroendocrine Function by Clemett, D. A., Cockett, M. I., Marsden, C. A., Fone, K. C. F.

“…: The effect of a 5‐hydroxytryptamine7 (5‐HT7) receptor‐directed antisense oligonucleotide on rat behaviour and neuroendocrine function was investigated. Six…”
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The C-terminal domain of 72 kDa gelatinase A is not required for catalysis, but is essential for membrane activation and modulates interactions with tissue inhibitors of metalloproteinases by Murphy, G, Willenbrock, F, Ward, R V, Cockett, M I, Eaton, D, Docherty, A J

“…Recombinant 72 kDa gelatinase A and a truncated form lacking the C-terminal domain were shown to be activated by organomercurials and to possess similar…”
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The activity of the tissue inhibitors of metalloproteinases is regulated by C-terminal domain interactions: A kinetic analysis of the inhibition of gelatinase A by Willenbrock, Frances, Crabbe, Thomas, Slocombe, Patrick M, Sutton, Chris W, Docherty, Andrew J. P, Cockett, Mark I, O'Shea, Mark, Brocklehurst, Keith, Phillips, Ian R, Murphy, Gillian

“…The cloning and expression of the full-length tissue inhibitor of metalloproteinase 2 (TIMP-2), delta 187-194TIMP-2, and delta 128-194TIMP-2 and the…”
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The N-terminal domain of tissue inhibitor of metalloproteinases retains metalloproteinase inhibitory activity by Murphy, Gillian, Houbrechts, Annick, Cockett, Mark I, Williamson, Richard A, O'Shea, Mark, Docherty, Andrew J. P

“…Recombinant tissue inhibitor of metalloproteinases (TIMP-1) and a truncated version containing only the three N-terminal loops, delta 127-184TIMP, have been…”
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Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes by Murphy, G, Cockett, M I, Stephens, P E, Smith, B J, Docherty, A J

“…The latent forms of stromelysin and collagenase from human gingival fibroblasts were purified to homogeneity. These latent proenzymes underwent serial small…”
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The construction of a highly efficient and versatile set of mammalian expression vectors by STEPHENS, P. E, COCKETT, M. I

“…The authors have developed a set of highly efficient and versatile vectors for the expression of heterologous in mammalian cells. These vectors allow the rapid…”
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Different Domain Interactions Are Involved in the Binding of Tissue Inhibitors of Metalloproteinases to Stromelysin-1 and Gelatinase A by Nguyen, Quang, Willenbrock, Frances, Cockett, Mark I, O'Shea, Mark, Docherty, Andrew J. P, Murphy, Gillian

“…The matrix metalloproteinases gelatinase A and stromelysin-1 have definable N-terminal (catalytic) and C-terminal domains. In order to analyze their…”
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Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors by Cockett, M I, Ochalski, R, Benwell, K, Franco, R, Wardwell-Swanson, J

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Site-directed mutations that alter the inhibitory activity of the tissue inhibitor of metalloproteinases-1: importance of the N-terminal region between cysteine 3 and cysteine 13 by O'Shea, Mark, Willenbrock, Frances, Williamson, Richard A, Cockett, Mark I, Freedman, Robert B, Reynolds, John J, Docherty, Andrew J. P, Murphy, Gillian

“…The tissue inhibitor of metalloproteinases-1 (TIMP-1) was subjected to single-site mutations within the N-terminal three loops using an…”
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Metalloproteinase domain structure, cellular invasion and metastasis by Cockett, M I, Birch, M L, Murphy, G, Hart, I R, Docherty, A J

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Selective Uncoupling of RGS Action by a Single Point Mutation in the G Protein α-Subunit by DiBello, Paul R., Garrison, Tiffany Runyan, Apanovitch, Donald M., Hoffman, Ginger, Shuey, David J., Mason, Kimberly, Cockett, Mark I., Dohlman, Henrik G.

“…Heterotrimeric G proteins function as molecular relays, shuttling between cell surface receptors and intracellular effectors that propagate a signal. G protein…”
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Crystallization and preliminary X-ray analysis of nonglycosylated tissue inhibitor of metalloproteinases-1, N30QN78Q TIMP-1 by Tolley, S P, Davies, G J, O'Shea, M, Cockett, M I, Docherty, A J, Murphy, G

“…A nonglycosylated (N30QN78Q) form of the human tissue inhibitor of metalloproteinases, TIMP-1, has been prepared and crystallized in a form suitable for X-ray…”
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New Generation Dopaminergic Agents. 1. Discovery of a Novel Scaffold Which Embraces the D2 Agonist Pharmacophore. Structure−Activity Relationships of a Series of 2-(Aminomethyl)chromans by Mewshaw, Richard E, Kavanagh, Joseph, Stack, Gary, Marquis, Karen L, Shi, Xiaojie, Kagan, Michael Z, Webb, Michael B, Katz, Alan H, Park, Anna, Kang, Young H, Abou-Gharbia, Magid, Scerni, Rosemary, Wasik, Theodore, Cortes-Burgos, Luz, Spangler, Taylor, Brennan, Julie A, Piesla, Michael, Mazandarani, Hossein, Cockett, Mark I, Ochalski, Rafal, Coupet, Joseph, Andree, Terrance H

“…A series of 2-(aminomethyl)chromans (2-AMCs) was synthesized and evaluated for their affinity and selectivity for both the high- and low-affinity agonist…”
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RGS7 attenuates signal transduction through the G(alpha q) family of heterotrimeric G proteins in mammalian cells by Shuey, D J, Betty, M, Jones, P G, Khawaja, X Z, Cockett, M I

“…The RGS proteins are a recently discovered family of G protein regulators that have been shown to act as GTPase-activating proteins (GAPs) on the G(alpha i)…”
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