Epitope mapping of cytochrome P450 cholesterol side-chain cleavage enzyme by sera from patients with autoimmune polyglandular syndrome type 1

Autoimmune polyglandular syndrome type 1 (APS-1) is a disease associated with defects of the autoimmune regulator gene and is characterized by autoimmune lesions of several tissues, predominantly endocrine glands, with multiple autoantibodies. In this study we describe autoantigenic epitopes on chol...

Full description

Saved in:

Bibliographic Details
Published in:	European journal of endocrinology Vol. 146; no. 1; pp. 113 - 119
Main Authors:	LIIV, Ingrid, TEESALU, Kaupo, PETERSON, Pärt, CLEMENTE, Maria Gracia, PERHEENTUPA, Jaakko, UIBO, Raivo
Format:	Journal Article
Language:	English
Published:	Colchester Portland Press 2002
Subjects:	Adolescent Adult Autoantibodies - analysis Biological and medical sciences Child Cholesterol - metabolism Cholesterol Side-Chain Cleavage Enzyme - blood Cholesterol Side-Chain Cleavage Enzyme - immunology Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Epitope Mapping Etiopathogenesis. Screening. Investigations. Target tissue resistance Female Female genital diseases Finland Gene Deletion Gynecology. Andrology. Obstetrics Humans Immunoblotting Immunoglobulin G - chemistry Immunoglobulin G - classification Italy Male Medical sciences Polyendocrinopathies, Autoimmune - blood Polyendocrinopathies, Autoimmune - enzymology Polyendocrinopathies, Autoimmune - immunology Tumors Italy Finland Human Endocrine gland Multiple Antigenic determinant Enzyme Cytochrome P450 Autoantibody Regulator gene Cholesterol Tissue Immunoblotting assay Cleavage Serum
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Autoimmune polyglandular syndrome type 1 (APS-1) is a disease associated with defects of the autoimmune regulator gene and is characterized by autoimmune lesions of several tissues, predominantly endocrine glands, with multiple autoantibodies. In this study we describe autoantigenic epitopes on cholesterol side-chain cleavage enzyme (P450scc) using sera from Finnish and Sardinian patients with APS-1, and analyze the epitope reactivities during disease follow-up. A series of P450scc cDNA fragments were expressed in E. coli and tested by immunoblotting assay using the patients' sera. Epitope regions were found over the whole P450scc molecule except the last N- (amino acids (aa) 1-40) and C-termini (aa 456-521). The strongest reactivity with patients' sera was found with central and C-terminal regions of the P450scc protein. All studied patients had IgG1 subclass antibodies. The results show that Finnish and Sardinian patients with APS-1 have similar, polyclonal immune reactions against P450scc, and that epitope reactivities did not change during the disease course. These results support the opinion that autoantibodies against P450scc and their epitope reactivity pattern are formed at an early stage of steroidogenic autoimmunity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0804-4643 1479-683X
DOI:	10.1530/eje.0.1460113