Correlation Between Inflammatory Cells and Sulfated Glycosaminoglycan Concentration in Synovial Fluid of Subjects with Secondary Knee Osteoarthritis

Objective To analyze the presence of inflammatory cells in the synovial fluid (SF) of subjects with osteoarthritis (OA) and correlate them with the concentration of sulfated glycosaminoglycan (GAG). Methods The degree of chondral injury from 20 subjects (17 men, 3 women, mean age 48.06 Â± 14.35 yrs)...

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Published in:	Journal of rheumatology Vol. 35; no. 6; pp. 1096 - 1101
Main Authors:	FRANCO, Renata N, CINTRA NETO, Paulo F. A, PIMENTEL, Edson R, COHEN, Moisés, LIMA, Geovane E. G, MATTIELLO-ROSA, Stela M. G
Format:	Journal Article
Language:	English
Published:	Toronto, ON The Journal of Rheumatology 01-06-2008 Journal of Rheumatology Publishing
Subjects:	Adult Biological and medical sciences Biomarkers Cartilage, Articular - injuries Cartilage, Articular - metabolism Cohort Studies Diseases of the osteoarticular system Female Glycosaminoglycans - analysis Humans Knee Injuries - metabolism Knee Injuries - pathology Leukocytes, Mononuclear Male Medical sciences Middle Aged Miscellaneous. Osteoarticular involvement in other diseases Neutrophils Osteoarthritis Osteoarthritis, Knee - etiology Synovial Fluid - chemistry Synovial Fluid - cytology Knee Human Knee osteoarthritis Diseases of the osteoarticular system Rheumatology Concentration Synovial fluid MONONUCLEAR AND POLYMORPHONUCLEAR LEUKOCYTES Arthropathy Glycosaminoglycan Degenerative disease OSTEOARTHRITIS;INFLAMMATION;SULFATED GLYCOSAMINOGLYCANS Inflammatory cell Neutrophil Osteoarthritis
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Summary:	Objective To analyze the presence of inflammatory cells in the synovial fluid (SF) of subjects with osteoarthritis (OA) and correlate them with the concentration of sulfated glycosaminoglycan (GAG). Methods The degree of chondral injury from 20 subjects (17 men, 3 women, mean age 48.06 Â± 14.35 yrs) was evaluated macroscopically by means of arthroscopy and graded according to the International Cartilage Repair Society classification. All subjects presented anterior cruciate ligament (ACL) injuries, associated or not to menisci injuries. SF was aspirated and centrifuged. Sulfated GAG concentration was quantified by dimethylene blue staining. Manual morphometry was used to determine the inflammatory cell count (mononuclear and polymorphonuclear) by optical microscopy, after staining with May-GrÃ¼nwald-Giemsa. The time of injury and degree of chondral injury were considered, and correlations among the variables were obtained by Spearman nonparametric correlation test. Results There was no significant correlation between the amount of mononuclear cells and the GAG concentration. There was a significant positive correlation between the polymorphonuclear cells and the GAG concentration, and a significant negative correlation between the degree of chondral injury and the GAG concentration. Conclusion Polymorphonuclear cells may be either a cause or an effect in the metabolic and biochemical processes occurring in chondral injuries. The correlations support the hypothesis that inflammatory cells have a significant role in the progression and chronicity of chondral injury in secondary OA. Key Indexing Terms: OSTEOARTHRITIS INFLAMMATION SULFATED GLYCOSAMINOGLYCANS MONONUCLEAR AND POLYMORPHONUCLEAR LEUKOCYTES
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0315-162X 1499-2752