Search Results - "CIGAN, A. Mark"

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes by Svitashev, Sergei, Schwartz, Christine, Lenderts, Brian, Young, Joshua K., Mark Cigan, A.

“…Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several…”
Get full text

Superior field performance of waxy corn engineered using CRISPR–Cas9 by Gao, Huirong, Gadlage, Mark J., Lafitte, H. Renee, Lenderts, Brian, Yang, Meizhu, Schroder, Megan, Farrell, Jeffry, Snopek, Kay, Peterson, Dave, Feigenbutz, Lanie, Jones, Spencer, St Clair, Grace, Rahe, Melissa, Sanyour-Doyel, Nathalie, Peng, Chenna, Wang, Lijuan, Young, Joshua K., Beatty, Mary, Dahlke, Brian, Hazebroek, Jan, Greene, Thomas W., Cigan, A. Mark, Chilcoat, N. Doane, Meeley, R. Bob

“…We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than…”
Get full text

The heterochronic maize mutant Corngrass1 results from overexpression of a tandem microRNA by Cigan, A Mark, Saeteurn, Koy, Hake, Sarah, Chuck, George

“…Retention of juvenile traits in the adult reproductive phase characterizes a process known as neoteny, and speculation exists over whether it has contributed…”
Get full text

Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding by Tucker, Elise J., Baumann, Ute, Kouidri, Allan, Suchecki, Radoslaw, Baes, Mathieu, Garcia, Melissa, Okada, Takashi, Dong, Chongmei, Wu, Yongzhong, Sandhu, Ajay, Singh, Manjit, Langridge, Peter, Wolters, Petra, Albertsen, Marc C., Cigan, A. Mark, Whitford, Ryan

“…The current rate of yield gain in crops is insufficient to meet the predicted demands. Capturing the yield boost from heterosis is one of the few technologies…”
Get full text

Male Fertility Genes in Bread Wheat (Triticum aestivum L.) and Their Utilization for Hybrid Seed Production by Singh, Manjit, Albertsen, Marc C., Cigan, A. Mark

“…Hybrid varieties can provide the boost needed to increase stagnant wheat yields through heterosis. The lack of an efficient hybridization system, which can…”
Get full text

MS26/CYP704B is required for anther and pollen wall development in bread wheat (Triticum aestivum L.) and combining mutations in all three homeologs causes male sterility by Singh, Manjit, Kumar, Manish, Thilges, Katherine, Cho, Myeong-Je, Cigan, A Mark

“…Development of anthers and pollen represents an important aspect of the life cycle in flowering plants. Genes contributing to anther and pollen development…”
Get full text

Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion by Gao, Huirong, Mutti, Jasdeep, Young, Joshua K, Yang, Meizhu, Schroder, Megan, Lenderts, Brian, Wang, Lijuan, Peterson, Dave, St Clair, Grace, Jones, Spencer, Feigenbutz, Lanie, Marsh, Wally, Zeng, Min, Wagner, Susan, Farrell, Jeffry, Snopek, Kay, Scelonge, Chris, Sopko, Xiaoyi, Sander, Jeffry D, Betts, Scott, Cigan, A Mark, Chilcoat, N Doane

“…Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing…”
Get full text

Technical considerations towards commercialization of porcine respiratory and reproductive syndrome (PRRS) virus resistant pigs by Mark Cigan, A., Knap, Pieter W.

“…Abstract The selection and introduction of disease resistance genes in livestock not only provide health benefits to animals but opportunities for breeders and…”
Get full text

A dominant mutation in mediator of paramutation2, one of three second-largest subunits of a plant-specific RNA polymerase, disrupts multiple siRNA silencing processes by Sidorenko, Lyudmila, Dorweiler, Jane E, Cigan, A Mark, Arteaga-Vazquez, Mario, Vyas, Meenal, Kermicle, Jerry, Jurcin, Diane, Brzeski, Jan, Cai, Yu, Chandler, Vicki L

“…Paramutation involves homologous sequence communication that leads to meiotically heritable transcriptional silencing. We demonstrate that mop2 (mediator of…”
Get full text

Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean by Betts, Scott D., Basu, Sutirtha, Bolar, Joy, Booth, Russ, Chang, Shujun, Cigan, A. Mark, Farrell, Jeffry, Gao, Huirong, Harkins, Kristi, Kinney, Anthony, Lenderts, Brian, Li, Zhongsen, Liu, Lu, McEnany, Michelle, Mutti, Jasdeep, Peterson, Dave, Sander, Jeffry D., Scelonge, Chris, Sopko, Xiaoyi, Stucker, Dave, Wu, Emily, Chilcoat, N. Doane

“…Development of transgenic cell lines or organisms for industrial, agricultural, or medicinal applications involves inserting DNA into the target genome in a…”
Get full text

Transcriptional gene silencing as a tool for uncovering gene function in maize by Cigan, A.M, Unger-Wallace, E, Haug-Collet, K

“…Transcriptional gene silencing has broad applications for studying gene function in planta. In maize, a large number of genes have been identified as…”
Get full text

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA by Svitashev, Sergei, Young, Joshua K., Schwartz, Christine, Gao, Huirong, Falco, S. Carl, Cigan, A. Mark

“…Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific insertion of a trait gene using clustered regularly interspaced short…”
Get full text

Cas9-Guide RNA Directed Genome Editing in Soybean by Li, Zhongsen, Liu, Zhan-Bin, Xing, Aiqiu, Moon, Bryan P., Koellhoffer, Jessica P., Huang, Lingxia, Ward, R. Timothy, Clifton, Elizabeth, Falco, S. Carl, Cigan, A. Mark

“…Recently discovered bacteria and archaea adaptive immune system consisting of clustered regularly interspaced short palindromic repeats (CRISPR) and…”
Get full text

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.) by Singh, Manjit, Kumar, Manish, Albertsen, Marc C., Young, Joshua K., Cigan, A. Mark

“…Key message Hexaploid bread wheat is not readily amenable to traditional mutagenesis approaches. In this study, we show efficient utilization of CRISPR-Cas…”
Get full text

Transcriptional gene silencing in bread wheat (Triticum aestivum L.) and its application to regulate male fertility for hybrid seed production by Singh, Manjit, Kumar, Manish, Califf, Kara E., Cigan, A. Mark

“…Summary Transcriptional gene silencing (TGS) can offer a straightforward tool for functional analysis of plant genes, particularly in polyploid species such as…”
Get full text

Development of a novel recessive genetic male sterility system for hybrid seed production in maize and other cross‐pollinating crops by Wu, Yongzhong, Fox, Tim W, Trimnell, Mary R, Wang, Lijuan, Xu, Rui‐ji, Cigan, A. Mark, Huffman, Gary A, Garnaat, Carl W, Hershey, Howard, Albertsen, Marc C

“…We have developed a novel hybridization platform that utilizes nuclear male sterility to produce hybrids in maize and other cross‐pollinating crops. A key…”
Get full text

Wheat ms5 male‐sterility is induced by recessive homoeologous A and D genome non‐specific lipid transfer proteins by Pallotta, Margaret A., Warner, Patricia, Kouidri, Allan, Tucker, Elise J., Baes, Mathieu, Suchecki, Radoslaw, Watson‐Haigh, Nathan, Okada, Takashi, Garcia, Melissa, Sandhu, Ajay, Singh, Manjit, Wolters, Petra, Albertsen, Marc C., Cigan, A. Mark, Baumann, Ute, Whitford, Ryan

“…Summary Nuclear male‐sterile mutants with non‐conditional, recessive and strictly monogenic inheritance are useful for both hybrid and conventional breeding…”
Get full text

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA by Svitashev, Sergei, Young, Joshua K., Schwartz, Christine, Gao, Huirong, Falco, S. Carl, Cigan, A. Mark

Get full text

Targeted mutagenesis of a conserved anther‐expressed P450 gene confers male sterility in monocots by Cigan, A. Mark, Singh, Manjit, Benn, Geoffrey, Feigenbutz, Lanie, Kumar, Manish, Cho, Myeong‐Je, Svitashev, Sergei, Young, Joshua

“…Summary Targeted mutagenesis using programmable DNA endonucleases has broad applications for studying gene function in planta and developing approaches to…”
Get full text

Cas9-Guide RNA Directed Genome Editing in Soybean by Li, Zhongsen, Liu, Zhan-Bin, Xing, Aiqiu, Moon, Bryan P., Koellhoffer, Jessica P., Huang, Lingxia, Ward, R. Timothy, Clifton, Elizabeth, Falco, S. Carl, Cigan, A. Mark

Get full text