Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach

Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken d...

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Published in:	Anais da Academia Brasileira de Ciências Vol. 93; no. 2; p. e20190632
Main Authors:	SILVA, CAIO C.A.R., GOMES, CAMILA L., DANDA, LUCAS J.A., ROBERTO, ANA EMÍLIA M., CARVALHO, ANA MARIA R. DE, XIMENES, EULÁLIA C.P.A., SILVA, ROSALI M.F. DA, ANGELOS, MATHEUS A., ROLIM, LARISSA A., ROLIM NETO, PEDRO J.
Format:	Journal Article
Language:	English
Published:	Academia Brasileira de Ciências 01-01-2021
Subjects:	Antibacterial antifungal experimental design microwave-assisted extraction MULTIDISCIPLINARY SCIENCES Syzigium cumini Syzigium cumini antifungal experimental design microwave-assisted extraction Antibacterial
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Summary:	Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken design that consisted of 27 experimental runs coupled with a desirability function for multiple response optimization was employed to optimize the total polyphenols content and total tannins content. Antimicrobial sensitivity tests were evaluated by obtaining the minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration in 96-well petri dishes. The optimal extraction conditions were found to be 8 min of extraction, under 300 w of microwave power, using a 1:34 g/mL solid/solvent ratio and 38% of ethanol concentration as extraction solvent. The parameter with the greatest influence in the extraction was primarily the time, followed by the potency and proportion solid/solvent. This yielded a total polyphenol content of 87.37 ± 1.85 mg TAE g-1ext and a total tannin content of 79.68 ± 1.64 mg TAE g-1 ext. All tested microorganisms were sensitive to the extract, evidencing the effectiveness of the extraction method optimization.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-3765 1678-2690 1678-2690
DOI:	10.1590/0001-3765202120190632