Amplification of repetitive DNA from Nicotiana plumbaginifolia in asymmetric somatic hybrids between Nicotiana sylvestris and Nicotiana plumbaginifolia

Amplification of repetitive DNA from Nicotiana plumbaginifolia in asymmetric somatic hybrids between Nicotiana sylvestris and Nicotiana plumbaginifolia

Asymmetric somatic hybrids were obtained between a chlorophyll- deficient mutant of Nicotiana sylvestris (V42) and a nitrate- reductase (NR)-deficient line of Nicotiana plumbaginifolia (cnx20 of Nia26), using each of the parents alternately as the irradiated donor. Irradiation doses applied ranged f...

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Bibliographic Details
Published in:Theoretical and applied genetics Vol. 86; no. 2-3; pp. 221 - 228
Main Authors: Kovtun, Y.V. (Ukrainian Academy of Sciences, Kiev (Ukraine). Inst. of Cell Biology and Genetic Engineering), Korostash, M.A, Butsko, Y.V, Gleba, Y.Y
Format: Journal Article
Language:English
Published: Heidelberg Springer 01-04-1993
Berlin
Subjects:
ADN
Asymmetric somatic hybrids
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA amplification
ESPECE
ESPECIES
Fundamental and applied biological sciences. Psychology
Gamma irradiation
Genetics of eukaryotes. Biological and molecular evolution
HIBRIDO SOMATICO
HYBRIDE SOMATIQUE
IRRADIACION GAMMA
IRRADIATION GAMMA
NICOTIANA
Nicotiana plumbaginifolia
Nicotiana sylvestris
Pteridophyta, spermatophyta
Species specific repetitive DNA
Tobacco
Vegetals
Genome organization
Genetic transformation
Repeated sequence
Chromosomic number
Gene amplification
Gamma irradiation
Nicotiana plumbaginifolia
Dicotyledones
Angiospermae
Interspecific hybridization
Somatic hybrid
Spermatophyta
Solanaceae
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Summary:Asymmetric somatic hybrids were obtained between a chlorophyll- deficient mutant of Nicotiana sylvestris (V42) and a nitrate- reductase (NR)-deficient line of Nicotiana plumbaginifolia (cnx20 of Nia26), using each of the parents alternately as the irradiated donor. Irradiation doses applied ranged from 10 to 1000 Gy of gamma-rays. Hybrid selection was based on complementation of NR deficiency with wild-type NR genes. To aid in the analysis somatic hybrids, species-specific repetitive DNA sequences from N. plumbaginifolia (NPR9 and NPR18) were cloned. NPR18 is a dispersed repetitive sequence occupying about 0,4% of the N. plumbaginifolia genome. In turn, NPR9, which is part of a highly repetitive DNA sequence, occupies approximately 3% of the genome. The species- specific plant DNA repeats, together with cytological analysis data, were used to assess the relative amount of the N. plumbaginifolia genome in the somatic hybrids. In fusion experiments using irradiated N. plumbaginifolia, an increase in irradiation dose prior to fusion led to a decrease in N. plumbaginifolia nuclear DNA content per hybrid genome. For some hybrid lines, an increase in the quantity of repetitive sequences was detected. Thus, hybrid lines 1NV/21, 100NV/7, 100NV/9, and 100NV (10 (where N. plumbaginifolia was the irradiated donor) were characterized by amplification of NPR9. In the reverse combination (where N. sylvestris was the irradiated donor), an increase in the copy number of NPR18 was determined for hybrid clones 1VC/2, 1VC/3, 100VC/2 and oct100/7. Possible reasons for the amplification of the repeated sequences are discussed
Bibliography:F30
93B0598
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ISSN:0040-5752
1432-2242
DOI:10.1007/BF00222082