Expression of monocyte chemoattractant protein-1 and interleukin-8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential

The differential expression of chemokine receptors may be an important mechanism for the regulation of T cell migration. To test this, we examined the expression and function of the monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 receptors on various population of T cells. Using a si...

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Bibliographic Details
Published in:	European journal of immunology Vol. 26; no. 3; p. 640
Main Authors:	Qin, S, LaRosa, G, Campbell, J J, Smith-Heath, H, Kassam, N, Shi, X, Zeng, L, Buthcher, E C, Mackay, C R
Format:	Journal Article
Language:	English
Published:	Germany 01-03-1996
Subjects:	Antibodies, Monoclonal - biosynthesis Antigens, CD - biosynthesis Antigens, CD - immunology Base Sequence Chemokine CCL2 - pharmacology Chemokine CCL4 Chemokine CCL5 - pharmacology Chemotaxis, Leukocyte - immunology Endothelium, Vascular - immunology Endothelium, Vascular - physiology Humans Immunophenotyping Interphase - drug effects Interphase - immunology Lymphocyte Activation - drug effects Macrophage Inflammatory Proteins Molecular Sequence Data Monokines - pharmacology Receptors, CCR2 Receptors, Chemokine Receptors, Cytokine - biosynthesis Receptors, Cytokine - immunology Receptors, Interleukin - biosynthesis Receptors, Interleukin - immunology Receptors, Interleukin-8A T-Lymphocyte Subsets - drug effects T-Lymphocyte Subsets - metabolism Up-Regulation
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Summary:	The differential expression of chemokine receptors may be an important mechanism for the regulation of T cell migration. To test this, we examined the expression and function of the monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 receptors on various population of T cells. Using a simple and reliable transendothelial chemotaxis assay, both MCP-1 and IL-8 were shown to be chemotactic for subsets of blood T cells, although the relative response varied from donor to donor. To examine receptor expression and correlate it with chemotaxis of T cell subsets, monoclonal antibodies (mAb) to the receptors were produced by immunizing mice either with synthetic peptides (MCP-1 receptor), or with receptor transfectants (IL-8 receptors A and B). A flow cytometric analysis of blood T cells with an anti-MCP-1 receptor mAb revealed low expression on the CD26hi subset and undetectable expression on other T cells. Staining of T cells with anti-Il-8RA and anti-IL-8RB showed much higher levels of expression, but only on a subset of CD3+ cells which were CD8+ and CD56+. That IL-8 and MCP-1 attracted distinct subsets of T cells was best illustrated using the CD26 marker, since IL-8R+ T cells were CD26-, whereas T cells expressing detectable MCP-1R or which responded to MCP-1 in chemotaxis assays were CD26hi. T cells activated in vitro with anti-CD3 up-regulated expression of the MCP-1 receptor, but not the IL-8 receptors, and were attracted to MCP-1 much more efficiently than resting T cells. These results show that there is a clear distinction between the IL-8 and MCP-1-responsive T cell populations and that chemokine receptor expression on T cells may be regulated with respect to linkage as well as cellular activation.
ISSN:	0014-2980
DOI:	10.1002/eji.1830260320