Search Results - "Busby, Stephen"

Local and global regulation of transcription initiation in bacteria by Browning, Douglas F., Busby, Stephen J. W.

“…Key Points Transcription initiation involves the interaction of DNA-dependent RNA polymerase with promoters. In bacteria, this is a highly regulated process…”
Get full text

Redefining fundamental concepts of transcription initiation in bacteria by Mejía-Almonte, Citlalli, Busby, Stephen J. W., Wade, Joseph T., van Helden, Jacques, Arkin, Adam P., Stormo, Gary D., Eilbeck, Karen, Palsson, Bernhard O., Galagan, James E., Collado-Vides, Julio

“…Despite enormous progress in understanding the fundamentals of bacterial gene regulation, our knowledge remains limited when compared with the number of…”
Get full text

Activating Transcription in Bacteria by Lee, David J, Minchin, Stephen D, Busby, Stephen J.W

“…Bacteria use a variety of mechanisms to direct RNA polymerase to specific promoters in order to activate transcription in response to growth signals or…”
Get full text

Transcription activation in bacteria: ancient and modern by Busby, Stephen J W

“…Regulatory interactions at the lac promoter.Activation of the transcription of genes is central to many processes of adaptation and differentiation in…”
Get full text

RNA polymerase spoiled for choice as transcription begins by Busby, Stephen J. W.

Get full text

Bacterial Transcription Factors: Regulation by Pick “N” Mix by Browning, Douglas F., Butala, Matej, Busby, Stephen J.W.

“…Transcription in most bacteria is tightly regulated in order to facilitate bacterial adaptation to different environments, and transcription factors play a key…”
Get full text

Escherichia coli “TatExpress” strains super‐secrete human growth hormone into the bacterial periplasm by the Tat pathway by Browning, Douglas F., Richards, Kirsty L., Peswani, Amber R., Roobol, Jo, Busby, Stephen J.W., Robinson, Colin

“…Numerous high‐value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec‐based production chassis cannot…”
Get full text

The regulation of bacterial transcription initiation by Busby, Stephen J. W, Browning, Douglas F

“…Bacteria use their genetic material with great effectiveness to make the right products in the correct amounts at the appropriate time. Studying bacterial…”
Get full text

Chromosome position effects on gene expression in Escherichia coli K-12 by Bryant, Jack A, Sellars, Laura E, Busby, Stephen J W, Lee, David J

“…In eukaryotes, the location of a gene on the chromosome is known to affect its expression, but such position effects are poorly understood in bacteria. Here,…”
Get full text

Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome by Grainger, David C, Hurd, Douglas, Goldberg, Martin D, Busby, Stephen J.W

“…The Escherichia coli chromosome is condensed into an ill-defined structure known as the nucleoid. Nucleoid-associated DNA-binding proteins are involved in…”
Get full text

Escherichia coli RutR transcription factor binds at targets within genes as well as intergenic regions by Shimada, Tomohiro, Ishihama, Akira, Busby, Stephen J.W, Grainger, David C

“…The Escherichia coli RutR protein is the master regulator of genes involved in pyrimidine catabolism. Here we have used chromatin immunoprecipitation in…”
Get full text

Evolution of bacterial transcription factors: how proteins take on new tasks, but do not always stop doing the old ones by Visweswariah, Sandhya S, Busby, Stephen J.W

“…Highlights • We suggest a pathway whereby bacterial transcription factors evolved from nucleoid-associated proteins whose original primary role was likely to…”
Get full text

Effects of nucleoid-associated proteins on bacterial chromosome structure and gene expression by Browning, Douglas F, Grainger, David C, Busby, Stephen JW

“…Bacterial nucleoid-associated proteins play a key role in the organisation, replication, segregation, repair and expression of bacterial chromosomes. Here, we…”
Get full text

Escherichia coli “TatExpress” strains export several g/L human growth hormone to the periplasm by the Tat pathway by Guerrero Montero, Isabel, Richards, Kirsty L., Jawara, Chillel, Browning, Douglas F., Peswani, Amber R., Labrit, Mickael, Allen, Matthew, Aubry, Cedric, Davé, Emma, Humphreys, David P., Busby, Stephen J. W., Robinson, Colin

“…Escherichia coli is a heavily used platform for the production of biotherapeutic and other high‐value proteins, and a favored strategy is to export the protein…”
Get full text

DNA barcodes for rapid, whole genome, single-molecule analyses by Wand, Nathaniel O, Smith, Darren A, Wilkinson, Andrew A, Rushton, Ashleigh E, Busby, Stephen J W, Styles, Iain B, Neely, Robert K

“…Abstract We report an approach for visualizing DNA sequence and using these ‘DNA barcodes’ to search complex mixtures of genomic material for DNA molecules of…”
Get full text

Tracking Low-Copy Transcription Factors in Living Bacteria: The Case of the lac Repressor by Garza de Leon, Federico, Sellars, Laura, Stracy, Mathew, Busby, Stephen J.W., Kapanidis, Achillefs N.

“…Transcription factors control the expression of genes by binding to specific sites in DNA and repressing or activating transcription in response to stimuli…”
Get full text

A method for targeting a specified segment of DNA to a bacterial microorganelle by Otoničar, Jan, Hostnik, Maja, Grundner, Maja, Kostanjšek, Rok, Gredar, Tajda, Garvas, Maja, Arsov, Zoran, Podlesek, Zdravko, Gostinčar, Cene, Jakše, Jernej, Busby, Stephen J W, Butala, Matej

“…Encapsulation of a selected DNA molecule in a cell has important implications for bionanotechnology. Non-viral proteins that can be used as nucleic acid…”
Get full text

Selective repression by Fis and H-NS at the Escherichia coli dps promoter by Grainger, David C, Goldberg, Martin D, Lee, David J, Busby, Stephen J.W

“…Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two…”
Get full text

A unified resource for transcriptional regulation in Escherichia coli K-12 incorporating high-throughput-generated binding data into RegulonDB version 10.0 by Santos-Zavaleta, Alberto, Sánchez-Pérez, Mishael, Salgado, Heladia, Velázquez-Ramírez, David A, Gama-Castro, Socorro, Tierrafría, Víctor H, Busby, Stephen J W, Aquino, Patricia, Fang, Xin, Palsson, Bernhard O, Galagan, James E, Collado-Vides, Julio

“…Our understanding of the regulation of gene expression has benefited from the availability of high-throughput technologies that interrogate the whole genome…”
Get full text

Inexpensive protein overexpression driven by the NarL transcription activator protein by Hothersall, Joanne, Lai, Sandie, Zhang, Nan, Godfrey, Rita E., Ruanto, Patcharawarin, Bischoff, Sarah, Robinson, Colin, Overton, Tim W., Busby, Stephen J. W., Browning, Douglas F.

“…Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple…”
Get full text