Comparison of Campylobacter jejuni PFGE and Penner subtypes in human infections and in water samples from the Taieri River catchment of New Zealand

Comparison of Campylobacter jejuni PFGE and Penner subtypes in human infections and in water samples from the Taieri River catchment of New Zealand

To determine the degree of overlap in strain types of Campylobacter jejuni isolated from clinical cases and water samples from the Taieri catchment in the South Island of New Zealand. Thermophilic Campylobacter were collected from human cases of infection, the main stem of the Taieri River and strea...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied microbiology Vol. 101; no. 1; pp. 18 - 25
Main Authors: Eyles, R.F, Brooks, H.J.L, Townsend, C.R, Burtenshaw, G.A, Heng, N.C.K, Jack, R.W, Weinstein, P
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-07-2006
Blackwell Science
Subjects:
Bacterial Typing Techniques
Biological and medical sciences
Campylobacter Infections - microbiology
Campylobacter Infections - transmission
Campylobacter jejuni
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
Campylobacter lari
campylobacteriosis
Disease Outbreaks
Disease Reservoirs
Electrophoresis, Gel, Pulsed-Field
Fresh Water
Fundamental and applied biological sciences. Psychology
human diseases
Humans
infection
land use
Microbiology
New Zealand
PFGE
polymerase chain reaction
Polymorphism, Restriction Fragment Length
pulsed-field gel electrophoresis
restriction endonucleases
risk
rivers
serotypes
Serotyping
streams
subtyping
surface water
water
Water Microbiology
watersheds
New Zealand
Oceania
Water
Human
Applied microbiology
Campylobacteraceae
Sample
Rivers
subtyping
Campylobacter jejuni
Infection
PFGE
Campylobacterjejuni
Bacteria
Subtype
Comparative study
Serotyping
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine the degree of overlap in strain types of Campylobacter jejuni isolated from clinical cases and water samples from the Taieri catchment in the South Island of New Zealand. Thermophilic Campylobacter were collected from human cases of infection, the main stem of the Taieri River and streams within distinct land-use types over a 1-year period. Campylobacter jejuni (187 isolates) and Campylobacter lari (four isolates) were identified using a multiplex polymerase chain reaction protocol. Isolates were typed by the Penner method and pulsed-field gel electrophoresis (PFGE) utilizing two restriction endonucleases. Several serotypes and PFGE types occurred in both water samples and clinical cases when the restriction profiles for each enzyme were considered separately. However, when PFGE profiles and serotyping were combined, there was no overlap between Camp. jejuni types from water and clinical cases. The results of this study indicate that recreational water in the Taieri catchment is not a major source of campylobacteriosis in the Dunedin area. This study suggests the risk of acquiring campylobacteriosis from surface waters in the Taieri catchment is considerably lower than previously predicted and highlights the necessity of using two endonucleases in PFGE typing.
Bibliography:http://dx.doi.org/10.1111/j.1365-2672.2006.02945.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2006.02945.x