Antimicrobial effect on some zoonotic bacteria, of the cell-free fermentation fluid and purified peptide fraction of the entomopathogenic bacterium, Xenorhabdus budapestensis

Antimicrobial effect on some zoonotic bacteria, of the cell-free fermentation fluid and purified peptide fraction of the entomopathogenic bacterium, Xenorhabdus budapestensis

Many multi-resistant patogens appear continuously resulting in a permanent need for the development of novel antibiotics. A large number of antibiotics introduced in clinical and veterinary practices are not effective. Antibacterial peptides with unusual mode of action may represent a promising opti...

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Bibliographic Details
Published in:Orvosi hetilap Vol. 156; no. 44; p. 1782
Main Authors: Burgettiné Böszörményi, Erzsébet, Barcs, István, Domján, Gyula, Bélafiné Bakó, Katalin, Fodor, András, Makrai, László, Vozik, Dávid
Format: Journal Article
Language:Hungarian
Published: Hungary 01-11-2015
Subjects:
Animals
Anti-Infective Agents - pharmacology
Cell-Free System
Colony Count, Microbial
Gram-Negative Bacteria - drug effects
Gram-Positive Bacteria - drug effects
Humans
Peptides - pharmacology
Xenorhabdus
Zoonoses - drug therapy
Zoonoses - microbiology
fabclavin
bicornutin-A
Xenorhabdus budapestensis
antimicrobial activity
antimikrobiális hatás
zoonosis
zoonózis
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Summary:Many multi-resistant patogens appear continuously resulting in a permanent need for the development of novel antibiotics. A large number of antibiotics introduced in clinical and veterinary practices are not effective. Antibacterial peptides with unusual mode of action may represent a promising option against multi-resistant pathogens. The entomopathogenic Xenorhabdus budapestensis bacteria produce several different antimicrobial peptides compounds such as bicornutin-A and fabclavin. The aim of the authors was to evaluate the in vitro antibacterial effect of Xenorhabdus budapestensis using zoonotic patogen bacteria. Cell-free conditioned media and purified peptide fractions of Xenorhabdus budapestensis were tested on Gram-positive (Rhodococcus equi, Erysipelothrix rhusiopathia, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, Listeria monocytagenes) and Gram-negative bacteria (Salmonella gallinarum, Salmonella derbi, Bordatella bronchoseptica, Escherichia coli, Pasteurella multocida, Aeromonas hydrophila) using agar diffusion test on blood agar plates. It was found that Xenorhabdus budapestensis bacteria produced compounds with strong and dose-dependent effects on the tested organisms. Purified peptid fraction exerted a more marked effect than cell free conditioned media. Gram-positive bacteria were more sensitive to this antibacterial effect than Gram-negative bacteria. Antibacterial peptide compound from Xenorhabdus budapestensis exert marked antibacterial effect on zoonotic patogen bacteria and they should be further evaluated in future for their potential use in the control or prevention of zoonoses.
ISSN:0030-6002
DOI:10.1556/650.2015.30274