Interactive gene expression pattern in prostate cancer cells exposed to phenolic antioxidants

Interactive gene expression pattern in prostate cancer cells exposed to phenolic antioxidants

Dietary phenolic compounds are known to elicite vital cellular responses such as cell cycle arrest, apoptosis and differentiation by activating a cascade of molecular events. As there is an increasing interest to improve the efficacy of these compounds for use as potential chemopreventive agents, we...

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) Vol. 70; no. 15; pp. 1821 - 1839
Main Authors: Narayanan, Bhagavathi A, Narayanan, Narayanan K, Stoner, Gary D, Bullock, Bryan P
Format: Journal Article
Language:English
Published: Netherlands Elsevier Inc 01-03-2002
Subjects:
Androgen
Anticarcinogenic Agents - pharmacology
Anticarcinogenic Agents - therapeutic use
Antioxidants - pharmacology
Antioxidants - therapeutic use
Carcinoma - drug therapy
Carcinoma - genetics
cDNA microarrays
DNA, Neoplasm - analysis
Ellagic acid
Ellagic Acid - pharmacology
Ellagic Acid - therapeutic use
Gene expression profiling
Gene Expression Profiling - methods
Gene Expression Regulation, Neoplastic - drug effects
Humans
LNCaP
Male
Oligonucleotide Array Sequence Analysis - methods
Phenols - pharmacology
Phenols - therapeutic use
Prostate
Prostatic Neoplasms - drug therapy
Prostatic Neoplasms - genetics
Resveratrol
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stilbenes - pharmacology
Stilbenes - therapeutic use
Transcription factors
Transmembrane proteins
Tumor Cells, Cultured - drug effects
Transcription factors
LNCaP
Androgen
Gene expression profiling
Ellagic acid
Resveratrol
Transmembrane proteins
Prostate
cDNA microarrays
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dietary phenolic compounds are known to elicite vital cellular responses such as cell cycle arrest, apoptosis and differentiation by activating a cascade of molecular events. As there is an increasing interest to improve the efficacy of these compounds for use as potential chemopreventive agents, we wanted to understand the impact of phenolic compounds on target genes in prostate cancer. In this study we used human cDNA microarrays with 2400 clones consisting of 17 prosite motifs to characterize alterations in gene expression pattern in response to the phenolic antioxidants ellagic acid (EA) and resveratrol (RE). Over a 48-hr exposure of androgen - sensitive LNCaP cells to EA and RE, a total of 593 and 555 genes respectively, showed more than a two fold difference in expression. A distinct set of genes in both EA-and RE-treated cells may represent the signature profile of phenolic antioxidant-induced gene expression in LNCaP cells. Although extensive similarity was found between effects of EA - and RE - responsive genes in prostate cancer cells, out of 246 genes with overlapping responses, 25 genes showed an opposite effect. Quantitative RT–PCR was used to verify and validate the differential expression of selected genes identified from cDNA microarrays. In-depth analysis of the data from this study provided insight into the alterations in the p53 - responsive genes, p300, Apaf–1, NF–kBp50 and p65 and PPAR families of genes, suggesting the activation of multiple signaling pathways that leads to growth inhibition of LNCaP cells. This is a first study to look for changes in a large number of human genes in response to dietary compounds.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(02)01481-9