Analysis of T-cell and B-cell responses to recombinant M. leprae antigens in leprosy patients and in healthy contacts: significant T-cell responses to antigens in M. leprae nonresponders

The recognition of a panel of recombinant Mycobacterium leprae antigens by T cells and B cells from 29 borderline tuberculoid/tuberculoid (BT/TT) and 18 lepromatous leprosy (LL) patients and from 21 healthy controls (HC) in leprosy-endemic regions of Ethiopia was examined. All 11 antigenic molecules...

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Bibliographic Details
Published in:	International journal of leprosy and other mycobacterial diseases Vol. 63; no. 3; p. 369
Main Authors:	Thole, J E, Janson, A A, Kifle, A, Howe, R C, McLean, K, Nurilygn, A, Filley, E, Shannon, E J, Bulla, G J, Hermans, J
Format:	Journal Article
Language:	English
Published:	United States 01-09-1995
Subjects:	Antibodies, Bacterial - blood Antigens, Bacterial - immunology B-Lymphocytes - immunology Bacterial Proteins - immunology Ethiopia Heat-Shock Proteins - immunology Humans Leprosy - immunology Leprosy, Borderline - immunology Leprosy, Lepromatous - immunology Leprosy, Tuberculoid - immunology Lymphocyte Activation Mycobacterium leprae - immunology Recombinant Fusion Proteins - immunology T-Lymphocytes - immunology Ethiopia
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Summary:	The recognition of a panel of recombinant Mycobacterium leprae antigens by T cells and B cells from 29 borderline tuberculoid/tuberculoid (BT/TT) and 18 lepromatous leprosy (LL) patients and from 21 healthy controls (HC) in leprosy-endemic regions of Ethiopia was examined. All 11 antigenic molecules tested (including M. leprae hsp 10, hsp18, hsp65 and several novel M. leprae antigens) were shown to be recognized by T cells, but clear quantitative differences existed between reactivities induced by individual antigens. Similar quantitative differences were observed when antibody responses to hsp10 and hsp65 antigens were determined. No associations were found between the antigen-specific responses and the subject status of either BT/TT and LL patients or HC. Fifteen percent of the patients who were nonresponsive to sonicates of M. leprae showed significant T-cell responses to one or more individual M. leprae antigens. This indicates that M. leprae constituents other than the proteins tested are responsible for the M. leprae-specific nonresponsiveness in these patients, which may be exploited for the design of vaccines or immunotherapeutic modalities aimed at inducing M. leprae-specific immunity in nonresponders.
ISSN:	0148-916X