Search Results - "Budin, Ghyslain"

Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals by Yevtodiyenko, Aleksey, Bazhin, Arkadiy, Khodakivskyi, Pavlo, Godinat, Aurelien, Budin, Ghyslain, Maric, Tamara, Pietramaggiori, Giorgio, Scherer, Sandra S., Kunchulia, Marina, Eppeldauer, George, Polyakov, Sergey V., Francis, Kevin P., Bryan, Jeffrey N., Goun, Elena A.

“…Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of…”
Get full text

A Magnetic Gram Stain for Bacterial Detection by Budin, Ghyslain, Chung, Hyun Jung, Lee, Hakho, Weissleder, Ralph

“…Magnetizing: Bacteria are often classified into Gram‐positive and Gram‐negative strains by staining with crystal violet (CV). The described bioorthogonal…”
Get full text

Engineered Trehalose Permeable to Mammalian Cells by Abazari, Alireza, Meimetis, Labros G, Budin, Ghyslain, Bale, Shyam Sundhar, Weissleder, Ralph, Toner, Mehmet

“…Trehalose is a naturally occurring disaccharide which is associated with extraordinary stress-tolerance capacity in certain species of unicellular and…”
Get full text

Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification by Budin, Ghyslain, Yang, Katherine S., Reiner, Thomas, Weissleder, Ralph

“…Click inside: A nuclear protein target, polo‐like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a…”
Get full text

Optimization of the Azobenzene Scaffold for Reductive Cleavage by Dithionite; Development of an Azobenzene Cleavable Linker for Proteomic Applications by Leriche, Geoffray, Budin, Ghyslain, Brino, Laurent, Wagner, Alain

“…In this paper we conducted an extensive reactivity study to determine the key structural features that favour the dithionite‐triggered reductive cleavage of…”
Get full text

Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions by Budin, Ghyslain, Dimala, Martin Moune, Lamour, Valérie, Oudet, Pierre, Mioskowski, Charles, Meunier, Stéphane, Brino, Laurent, Wagner, Alain

“…Extra‐mild nondenaturing protocol: Fishing for unaltered protein in a lysate is a prerequisite for identifying and analyzing the architecture of a protein…”
Get full text

A bioluminescent probe for longitudinal monitoring of mitochondrial membrane potential by Bazhin, Arkadiy A., Sinisi, Riccardo, De Marchi, Umberto, Hermant, Aurélie, Sambiagio, Nicolas, Maric, Tamara, Budin, Ghyslain, Goun, Elena A.

“…Mitochondrial membrane potential (Δ Ψ m ) is a universal selective indicator of mitochondrial function and is known to play a central role in many human…”
Get full text

Ubiquitous Detection of Gram-Positive Bacteria with Bioorthogonal Magnetofluorescent Nanoparticles by Chung, Hyun Jung, Reiner, Thomas, Budin, Ghyslain, Min, Changwook, Liong, Monty, Issadore, David, Lee, Hakho, Weissleder, Ralph

“…The ability to rapidly diagnose gram-positive pathogenic bacteria would have far reaching biomedical and technological applications. Here we describe the…”
Get full text

Bioorthogonal Imaging of Aurora Kinase A in Live Cells by Yang, Katherine S., Budin, Ghyslain, Reiner, Thomas, Vinegoni, Claudio, Weissleder, Ralph

“…In living color: Aurora kinase A (AKA) was imaged in live cells using a bioorthogonal two‐step reaction with a small molecule AKA inhibitor (see scheme) and a…”
Get full text

Bioorthogonal Approach to Identify Unsuspected Drug Targets in Live Cells by Yang, Katherine S., Budin, Ghyslain, Tassa, Carlos, Kister, Olivier, Weissleder, Ralph

“…A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans‐cyclooctene (TCO) and…”
Get full text

μHall Chip for Sensitive Detection of Bacteria by Issadore, David, Chung, Hyun Jung, Chung, Jaehoon, Budin, Ghyslain, Weissleder, Ralph, Lee, Hakho

“…Sensitive, rapid and phenotype‐specific enumeration of pathogens is essential for the diagnosis of infectious disease, monitoring of food chains, and for…”
Get full text

Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection by Wang, Yu, Thompson, John M., Ashbaugh, Alyssa G., Khodakivskyi, Pavlo, Budin, Ghyslain, Sinisi, Riccardo, Heinmiller, Andrew, van Oosten, Marleen, van Dijl, Jan Maarten, van Dam, Gooitzen M., Francis, Kevin P., Bernthal, Nicholas M., Dubikovskaya, Elena A., Miller, Lloyd S.

“…Introduction: Diagnosing prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or…”
Get full text

Bioorthogonal Probes for Polo-like Kinase1 Imaging and Quantification by Budin, Ghyslain, Yang, Katherine S, Reiner, Thomas, Weissleder, Ralph

Get full text

A FRET-based probe with a chemically deactivatable quencher by Leriche, Geoffray, Budin, Ghyslain, Darwich, Zeinab, Weltin, Denis, Mély, Yves, Klymchenko, Andrey S, Wagner, Alain

“…A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a…”
Get more information

Azidation of β-carbonyl lactones and lactams by Kashinath, Dhurke, Budin, Ghyslain, Baati, Rachid, Meunier, Stéphane, Wagner, Alain

“…The direct azidation of various heterocyclic β-ketoesters, lactones, and lactams is reported. By using tosylazide and an organic base such as l-proline or TBD,…”
Get full text

µHall Chip for Sensitive Detection of Bacteria by Issadore, David, Chung, Hyun Jung, Chung, Jaehoon, Budin, Ghyslain, Weissleder, Ralph, Lee, Hakho

“…Sensitive, rapid and phenotype-specific enumeration of pathogens is essential for the diagnosis of infectious disease, monitoring of food chains, and for…”
Get full text

Nondenaturing Chemical Proteomics for Protein Complex Isolation and Identification by Budin, Ghyslain, Moune‐Dimala, Martin, Leriche, Geoffray, Saliou, Jean‐Michel, Papillon, Julie, Sanglier‐Cianférani, Sarah, Van Dorsselaer, Alain, Lamour, Valérie, Brino, Laurent, Wagner, Alain

“…Controlled fishing: Mild cleavage conditions enabled by the use of the HAZA linker allowed smooth release of captured proteins under nondenaturing conditions…”
Get full text

A Magnetic Gram Stain for Bacterial Detection by Budin, Ghyslain, Chung, Hyun Jung, Lee, Hakho, Weissleder, Ralph

“…Magnetisierend: Bakterien werden durch Färbung mit Kristallviolett (CV) oft in Gram‐positive und Gram‐negative Stämme klassifiziert. Die bioorthogonale…”
Get full text

Bioorthogonal Approach to Identify Unsuspected Drug Targets in Live Cells by Yang, Katherine S., Budin, Ghyslain, Tassa, Carlos, Kister, Olivier, Weissleder, Ralph

“…Sekundäre Wirkstoff‐Targets werden mit der beschriebenen Methode in lebenden Zellen identifiziert. Der Wirkstoff wird mit trans‐Cycloocten (TCO) modifiziert…”
Get full text

Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification by Budin, Ghyslain, Yang, Katherine S., Reiner, Thomas, Weissleder, Ralph

“…Ein Zellkernprotein, die Polo‐like‐Kinase 1 (PLK1), wurde mithilfe einer biokompatiblen bioorthogonalen Ligation zwischen einem spezifischen Wirkstoff und…”
Get full text