Clinical value of DNA image cytometry in effusions with atypia

Malignant cells in serosal effusions provide essential information about the extent of malignant disease. The main aim of this study was to examine the additional diagnostic value of DNA image cytometry for cases with uncertainty in the cytological diagnosis. In addition, the feasibility of automate...

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Bibliographic Details
Published in:	Diagnostic cytopathology Vol. 21; no. 2; pp. 112 - 116
Main Authors:	Thunnissen, Frederik B.J.M., Buchholtz, Ruud T.F., Woutersen, Dankert P., Arends, Jan Willem, van der Putten, Hans W.H.M., ten Velde, Guul P.M.
Format:	Journal Article
Language:	English
Published:	New York John Wiley & Sons, Inc 01-08-1999 Wiley-Liss
Subjects:	Adult Aged Aged, 80 and over Aneuploidy Ascitic Fluid - pathology atypia Biological and medical sciences cytology diagnosis DNA cytometry DNA, Neoplasm - analysis effusions Granulocytes - pathology Humans Image Cytometry - methods Image Processing, Computer-Assisted Investigative techniques, diagnostic techniques (general aspects) Lymphocytes - pathology Medical sciences Middle Aged Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pericardial Effusion - pathology Pilot Projects Pleural Effusion - genetics Pleural Effusion - pathology Predictive Value of Tests Staining and Labeling Human Effusion Respiratory disease Immunocytochemistry Cardiovascular disease Pericardium Pathology Cytometry Abdominal disease DNA Pericardial disease Pleural disease Cytopathology Atypical Diagnosis Technique Pleura Peritoneum
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Summary:	Malignant cells in serosal effusions provide essential information about the extent of malignant disease. The main aim of this study was to examine the additional diagnostic value of DNA image cytometry for cases with uncertainty in the cytological diagnosis. In addition, the feasibility of automated nuclei detection was investigated. Out of 457 cases, 33 samples in 32 patients were diagnosed with “atypia” (probably benign) and 21 as “suspicious for malignancy.” DNA image cytometry was performed on these 54 cases and on an additional group of 14 cytologically malignant cases. The results show that automatic classification is useful for separation of control cells, i.e., lymphocytes and neutrophilic granulocytes from other mononuclear cells. In 21 cases an insufficient number of control cells were measured. Seventy‐two percent of the cytologic malignant cases were aneuploid. In contrast, in none of the cases with “atypia” and in only 2 of the cases “suspicious for malignancy” was aneuploidy present (2 of the remaining 32, 6%). From the cases with follow‐up, a malignancy in the pleural fluid was present in 2 out of 17 cases with “atypia” and in 5 out of 10 with a “suspicious for malignancy” cytologic diagnosis, respectively. In conclusion, the additional diagnostic value of DNA image cytometry in cases with a cytological diagnosis of “atypia” or “suspicious for malignancy” is limited. Diagn. Cytopathol. 1999;21:112–116. © 1999 Wiley‐Liss, Inc.
Bibliography:	istex:13FB6F44B80C2AB4D4C9CAD2A9BE0AC17E0F66B0 ArticleID:DC6 ark:/67375/WNG-V54LP72X-Z ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	8755-1039 1097-0339
DOI:	10.1002/(SICI)1097-0339(199908)21:2<112::AID-DC6>3.0.CO;2-D