Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada

Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada

Microsatellite DNA markers from 13 simple sequence repeat (SSR) loci were used to compare genetic diversity between preharvest pristine and postharvest residual gene pools of two adjacent virgin, old‐growth (≈ 250 years) stands of eastern white pine (Pinus strobus L.) in Ontario. There was concurren...

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Bibliographic Details
Published in:Molecular ecology Vol. 9; no. 3; pp. 339 - 348
Main Authors: Rajora, O.P, Rahman, M.H, Buchert, G.P, Dancik, B.P
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Science Ltd 01-03-2000
Subjects:
Alleles
allelic richness
Canada, Ontario
coniferous forests
DNA, Plant - genetics
Ecosystem
Forestry
gene conservation
gene frequency
gene pool
genetic diversity
genetic markers
Genetic Variation
Genetics, Population
heterozygosity
loci
logging
Microsatellite Repeats
natural regeneration
Ontario
Pinus strobus
rare alleles
repetitive sequences
silvicultural practices
silvicultural systems
simple sequence repeats
stand density
sustainable forest management
Trees - genetics
Ontario
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Summary:Microsatellite DNA markers from 13 simple sequence repeat (SSR) loci were used to compare genetic diversity between preharvest pristine and postharvest residual gene pools of two adjacent virgin, old‐growth (≈ 250 years) stands of eastern white pine (Pinus strobus L.) in Ontario. There was concurrence in genetic diversity changes in the postharvest gene pools of the two stands. The total and mean numbers of alleles detected in each stand were reduced by ≈ 26% after tree density reductions of ≈ 75%. Approximately 18 and 21% of the low‐frequency (0.25 > P ≥ 0.01) alleles and 76 and 92% of the rare (P < 0.01) alleles were lost from residual stands A and B, respectively, after harvesting. Multilocus gametic diversity was reduced by 38 and 85% and genotype additivity by ≈ 50% in the residual stands after harvesting. Latent genetic potential of each stand was reduced by ≈ 40%. Although heterozygosity was reduced (1–5%) in the postharvest residual stands, the reductions were not substantial and not comparable to those using other genetic diversity measures. The reductions in genetic diversity measures were slightly higher than those theoretically expected in postbottleneck populations according to drift theory. In the absence of substantial gene migration that could ameliorate the genetic losses, the ability of the postharvest white pine gene pools to adapt to changing environmental and disease conditions may have been compromised. The microsatellite DNA results for genetic effects of harvesting in old‐growth eastern white pine stands were similar to those that we reported earlier from allozyme analysis ( Buchert et al. 1997 ). The results indicate that silvicultural practices should ensure that the gene pools of remaining pristine old‐growth stands are reconstituted in the regenerating stands.
Bibliography:istex:998FE65FD6BA92CB6A9160B6A04D046CD6CD6CA0
ArticleID:MEC886
ark:/67375/WNG-BL57X96F-F
Present address: The Algoma Institute, 330 North 100 West, Provo, UT 84601, USA.
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ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294x.2000.00886.x