Identification and expression analysis of NOD-like receptor gene family in response to Aeromonas hydrophila and poly I:C challenge in Chinese soft-shelled turtle (Pelodiscus sinensis)

Nucleotide-binding domain (NOD)-like receptors (NLRs), a type of pattern recognition receptor (PRR), specifically recognize pathogen-associated molecular patterns and damage-associated molecular patterns, playing crucial roles in the immune system. Herein, the composition, expression pattern, and co...

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Bibliographic Details
Published in:	Aquaculture reports Vol. 38; p. 102312
Main Authors:	Cao, Yang, Feng, Rui, Jiao, Lixin, Bu, Lingjiao, Tian, Jianzhong, Wan, Haifu, Guan, Yueqiang
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-10-2024 Elsevier
Subjects:	Immune response Nucleotide-binding domain (NOD)-like receptor gene family Pelodiscus sinensis Weighted gene co-expression network analysis Pelodiscus sinensis Immune response Nucleotide-binding domain (NOD)-like receptor gene family Weighted gene co-expression network analysis
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Summary:	Nucleotide-binding domain (NOD)-like receptors (NLRs), a type of pattern recognition receptor (PRR), specifically recognize pathogen-associated molecular patterns and damage-associated molecular patterns, playing crucial roles in the immune system. Herein, the composition, expression pattern, and co-expressed pathways of NLRs in Pelodiscus sinensis (PsNLRs) were analyzed. Eight PsNLRs, located on seven chromosomes, were first identified from the genome. Phylogenetic analysis revealed that PsNLRs were most closely related to the NLRs of the western painted turtle and the green sea turtle, and could be clustered into four subfamilies. Besides, the expression profile of PsNLRs exhibited tissue-specificity after infection with Aeromonas hydrophila or poly I:C. Nonetheless, PsCIITA and PsNLRP3.2 displayed infection-specific expression patterns, whereas other PsNLRs showed similar expression patterns during A. hydrophila and poly I:C infections. Weighted gene co-expression network analysis found that six PsNLRs were generated in the turquoise module, in which several immune-related signaling pathways were enriched, including “NOD-like receptor signaling pathway”, “NF-κB signaling pathway”, “PI3K-Akt signaling pathway”, “Chemokine signaling pathway”, “Antigen processing and presentation”, and ”Toll-like receptor signaling pathway”. Additionally, three immune-related genes, NF-κB1, TRAF3, and PIK3CG, were identified as hub genes. These results indicated that PsNLRs may play key roles in regulating immune responses in P. sinensis. Overall, an in-depth study of the NLR gene family in P. sinensis would yield insights into developing strategies to combat bacterial and viral infections. •Eight NOD-like receptor genes were identified from Pelodiscus sinensis.•Six PsNLRs were co-expressed with several immune-related signaling pathways.•PsCIITA and PsNLRP3.2 displayed infection-specific expression patterns.•NF-κB1, TRAF3, and PIK3CG are key immune genes in response to different infections.
ISSN:	2352-5134 2352-5134
DOI:	10.1016/j.aqrep.2024.102312