Evaluation of the polarographic technique for assay of the viability of freeze-dried BCG vaccine: II. Viability of the vaccine assessed by polarography, Warburg respirometry and colony counting

A comparative study of the viability of 34 lots of freeze-dried BCG vaccine has been carried out using an oxygen electrode polarographic technique, Warburg respirometry and colony counting. There were no statistical differences between the results obtained with the polarographic and Warburg techniqu...

Full description

Saved in:

Bibliographic Details
Published in:	Vaccine Vol. 13; no. 3; pp. 273 - 275
Main Authors:	Malucelli, Maria Ivette Carboni, Niero, Rinaldo, Lucchiari, Pedro Helio, Souza, Magda D.C., Bruzzo, Dulcinéia, Alves, Rosely C.B., Miguel, Omar, Bacila, Metry
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 1995 Elsevier
Subjects:	Bacteriological methods and techniques used in bacteriology Bacteriology BCG Vaccine BCG viability Biological and medical sciences Colony Count, Microbial colony-counting technique Electrodes Evaluation Studies as Topic Freeze Drying Fundamental and applied biological sciences. Psychology Microbiology Mycobacterium Mycobacterium bovis - immunology Mycobacterium bovis - metabolism Mycobacterium bovis - physiology Oxygen - analysis Oxygen - pharmacokinetics Oxygen Consumption oxygen uptake polarographic method Polarography - methods Warburg method polarographic method oxygen uptake colony-counting technique BCG viability Warburg method Performance evaluation Freeze drying Rapid technique Polarography Respirometry BCG Vaccine Measurement method Viability Counting
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A comparative study of the viability of 34 lots of freeze-dried BCG vaccine has been carried out using an oxygen electrode polarographic technique, Warburg respirometry and colony counting. There were no statistical differences between the results obtained with the polarographic and Warburg techniques. Both methods gave reliable and similar results and showed a positive correlation ( r = 0.8615). Comparison between the polarographic and colony-counting methods showed a positive correlation ( r = 0.6530); for comparison between the Warburg and colony-counting methods, the correlation value was r = 0.6868. All the tests were significant at the level of α = 0.05. The advantages of the polarographic technique are that it is much less time- and material-consuming than other methods. It is a reliable, inexpensive and convenient method for BCG vaccine quality control.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0264-410X 1873-2518
DOI:	10.1016/0264-410X(95)93313-X