re-examination of succinic dehydrogenase activity and its association with cell death in the interdigit of the chick foot

ABSTRACT Massive cell death, which accompanies the freeing of the digits, occurs in the inter digits of the developing chick foot during stages 30–35. The possibility was reexamined that mitochondrial succinic dehydrogenase activity disappears in the interdigital cells of the chick foot 24 h before...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of cell science Vol. 15; no. 1; pp. 17 - 29
Main Authors:	Fallon, J.F, Brucker, R.F, Harris, C.M
Format:	Journal Article
Language:	English
Published:	England 01-06-1974
Subjects:	animal science Animals Cell Differentiation Cell Survival Chick Embryo - enzymology Chick Embryo - growth & development Cytochrome Reductases - metabolism Electron Transport Foot - cytology Foot - embryology Foot - enzymology livestock Mitochondria - enzymology NAD Succinate Dehydrogenase - metabolism zoology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	ABSTRACT Massive cell death, which accompanies the freeing of the digits, occurs in the inter digits of the developing chick foot during stages 30–35. The possibility was reexamined that mitochondrial succinic dehydrogenase activity disappears in the interdigital cells of the chick foot 24 h before cell mortality. Using standard histochemical means it was found that succinic dehydrogenase activity was demonstrable in the interdigital cell population up to and including the time when cell death begins. This was confirmed quantitatively employing biochemical methods which indicated that succinic-cytochrome c reductase specific activity was approximately the same in homogenates of digital and interdigital tissues harvested just prior to the death of interdigital cells. Further, NADH-cytochrome c reductase specific activity was comparable in digital and interdigital tissue homogenates. These data make it unlikely that ‘biochemical degeneration’ associated with alterations in aerobic energy metabolism occurs in the interdigital cells before death as has been suggested by other workers previously. The methods used by these authors to demonstrate succinic dehydrogenase were repeated on chick feet in our laboratories and an alternative interpretation of the results is offered.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9533 1477-9137
DOI:	10.1242/jcs.15.1.17