Search Results - "Broude, Natalia E"
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Ab initio RNA folding by discrete molecular dynamics: from structure prediction to folding mechanisms
Published in RNA (Cambridge) (01-06-2008)“…RNA molecules with novel functions have revived interest in the accurate prediction of RNA three-dimensional (3D) structure and folding dynamics. However,…”
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Analysis of RNA localization and metabolism in single live bacterial cells: achievements and challenges
Published in Molecular microbiology (01-06-2011)“…Summary Studies of gene expression at the single cell level in live bacterial cells represent a new and fertile area of research providing real‐time, spatially…”
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3
RNA visualization in live bacterial cells using fluorescent protein complementation
Published in Nature methods (01-05-2007)“…We describe a technique for the detection and localization of RNA transcripts in living cells. The method is based on fluorescent-protein complementation…”
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Labeling native bacterial RNA in live cells
Published in Cell research (01-07-2014)“…Labeling RNA molecules in their physiological environment is still a technological challenge that should be overcome to study kinetics, localization and…”
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Ultrasensitive detection of DNA and protein markers in cancer cells
Published in Cancer biology & medicine (01-09-2015)“…Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression…”
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Spatiotemporal patterns and transcription kinetics of induced RNA in single bacterial cells
Published in Proceedings of the National Academy of Sciences - PNAS (22-09-2009)“…Bacteria have a complex internal organization with specific localization of many proteins and DNA, which dynamically move during the cell cycle and in response…”
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7
GC/AT-Content Spikes as Genomic Punctuation Marks
Published in Proceedings of the National Academy of Sciences - PNAS (30-11-2004)“…Large-scale analysis of the GC-content distribution at the gene level reveals both common features and basic differences in genomes of different groups of…”
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Fast Complementation of Split Fluorescent Protein Triggered by DNA Hybridization
Published in Proceedings of the National Academy of Sciences - PNAS (14-02-2006)“…Fluorescent proteins have proven to be excellent reporters and biochemical sensors with a wide range of applications. In a split form, they are not…”
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RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides
Published in Pharmaceuticals (Basel, Switzerland) (01-03-2011)“…Many genetic and infectious diseases can be targeted at the RNA level as RNA is more accessible than DNA. We seek to develop new approaches for detection and…”
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Probing the Genetic Population Structure of Trypanosoma cruzi with Polymorphic Microsatellites
Published in Proceedings of the National Academy of Sciences - PNAS (31-03-1998)“…We describe here the identification of eight polymorphic microsatellite loci with (CA)nrepeats in the Trypanosoma cruzi genome based on the affinity capture of…”
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Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology
Published in Trends in Biotechnology (01-06-2002)“…The specific structural features of stem-loop (hairpin) DNA constructs provide increased specificity of target recognition. Recently, several robust assays…”
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Multiplex Allele-Specific Target Amplification Based on PCR Suppression
Published in Proceedings of the National Academy of Sciences - PNAS (02-01-2001)“…We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer…”
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Visualization of induced RNA in single bacterial cells
Published in Methods in molecular biology (Clifton, N.J.) (2011)“…Visualization of RNA in live cells is a challenging task due to the transient character of most RNA molecules and the lack of adequate methods to label RNA…”
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Enhanced DNA Sequencing by Hybridization
Published in Proceedings of the National Academy of Sciences - PNAS (12-04-1994)“…An enhanced version of DNA sequencing by hybridization (SBH), termed positional SBH (PSBH), has been developed. PSBH uses duplex probes containing…”
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15
Differential Display of Genome Subsets Containing Specific Interspersed Repeats
Published in Proceedings of the National Academy of Sciences - PNAS (29-04-1997)“…A genomic differential display method was developed that analyzes many restriction fragment length polymorphisms simultaneously. Interspersed repeat sequences…”
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Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro
Published in Nature protocols (01-07-2006)“…Here, we present a protocol for isolating the large N-terminal fragment of enhanced green fluorescent protein (EGFP) with a preformed chromophore. By itself,…”
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Trouble with differential display
Published in Trends in Biotechnology (01-04-2002)“…[...]a chapter on the microSAGE (serial analysis of gene expression) technique describes a modification from the original SAGE procedure adapted for gene…”
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Journal Article Book Review -
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Differential display in the time of microarrays
Published in Expert review of molecular diagnostics (01-05-2002)“…A brief overview of major methods used for genome-wide expression profiling is presented. Special attention is devoted to ordered differential display,…”
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Real-time monitoring of branched rolling-circle DNA amplification with peptide nucleic acid beacon
Published in Analytical biochemistry (15-12-2004)Get full text
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A cancer roadmap
Published in Expert review of molecular diagnostics (01-03-2005)Get more information
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