Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease

Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease

Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used by the organism to enhance or repress gene expression in response to stimuli. Here, we report on the genome-wide analysis of the Theobroma cacao WRKY TF family and also investigate th...

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Bibliographic Details
Published in:PloS one Vol. 12; no. 10; p. e0187346
Main Authors: Silva Monteiro de Almeida, Dayanne, Oliveira Jordão do Amaral, Daniel, Del-Bem, Luiz-Eduardo, Bronze Dos Santos, Emily, Santana Silva, Raner José, Peres Gramacho, Karina, Vincentz, Michel, Micheli, Fabienne
Format: Journal Article
Language:English
Published: United States Public Library of Science 30-10-2017
Public Library of Science (PLoS)
Subjects:
Abiotic stress
Arabidopsis
Arabidopsis thaliana
Cacao
Cacao - genetics
Chocolate
Chromosomes
Deoxyribonucleic acid
Diseases and pests
DNA
Gene expression
Gene regulation
Gene sequencing
Genes
Genetic aspects
Genomes
Genomics
Identification
Moniliophthora perniciosa
Oryza
Phylogenetics
Phylogeny
Physiological aspects
Plant diseases
Plant Diseases - genetics
Plant sciences
Proteins
Senescence
Subgroups
Theobroma cacao
Transcription factors
Transcription Factors - metabolism
Witches' broom
Zinc
Zinc finger proteins
Brazil
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Summary:Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used by the organism to enhance or repress gene expression in response to stimuli. Here, we report on the genome-wide analysis of the Theobroma cacao WRKY TF family and also investigate the expression of WRKY genes in cacao infected by the fungus Moniliophthora perniciosa. In the cacao genome, 61 non-redundant WRKY sequences were found and classified in three groups (I to III) according to the WRKY and zinc-finger motif types. The 61 putative WRKY sequences were distributed on the 10 cacao chromosomes and 24 of them came from duplication events. The sequences were phylogenetically organized according to the general WRKY groups. The phylogenetic analysis revealed that subgroups IIa and IIb are sister groups and share a common ancestor, as well as subgroups IId and IIe. The most divergent groups according to the plant origin were IIc and III. According to the phylogenetic analysis, 7 TcWRKY genes were selected and analyzed by RT-qPCR in susceptible and resistant cacao plants infected (or not) with M. perniciosa. Some TcWRKY genes presented interesting responses to M. perniciosa such as Tc01_p014750/Tc06_p013130/AtWRKY28, Tc09_p001530/Tc06_p004420/AtWRKY40, Tc04_p016130/AtWRKY54 and Tc10_p016570/ AtWRKY70. Our results can help to select appropriate candidate genes for further characterization in cacao or in other Theobroma species.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0187346