Search Results - "Broeze, R J"
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Characterization of a nonglycosylated single chain urinary plasminogen activator secreted from yeast
Published in The Journal of biological chemistry (15-01-1990)“…Using site-directed mutagenesis, we have changed the asparagine in human single-chain urinary plasminogen activator (u-PA) at position 302 to an alanine. This…”
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Effects of low temperature on in vivo and in vitro protein synthesis in Escherichia coli and Pseudomonas fluorescens
Published in Journal of Bacteriology (01-06-1978)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Homogeneous, liposome-based assay for total complement activity in serum
Published in Clinical chemistry (Baltimore, Md.) (01-02-1986)“…This is a rapid, homogeneous, liposome-based assay for total complement activity in human serum. Liposome-encapsulated enzyme is unmasked by the action of…”
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Fibrin specific thrombolysis by two-chain urokinase-type plasminogen activator cleaved after arginine 156 by thrombin
Published in Thrombosis and haemostasis (1990)“…Scu-PA was cleaved by thrombin after arginine-156 to yield a two-chain molecule with low amidolytic activity and resistance to cleavage by plasmin…”
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Spin Filter Perfusion System for High Density Cell Culture: Production of Recombinant Urinary Type Plasminogen Activator in CHO Cells
Published in Bio/technology (New York, N.Y. 1983) (01-01-1990)“…We have used a 20 liter stirred tank fermentor, equipped with a 127 mesh ethylene-tetrafluoroethylene rotating screen for cell recycle, for the continuous…”
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Large Scale Production of Mouse Interferons from Monolayers of Ehrlich Ascites Tumour Cells
Published in Journal of general virology (01-07-1980)“…Department of Molecular Biophysics and Biochemistry Yale University, New Haven Connecticut 06511, U.S.A. Conditions are described for the large scale…”
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Structural characteristics of interferons from mouse Ehrlich ascites tumor cells
Published in The Journal of biological chemistry (25-05-1979)“…An improved procedure for the isolation of interferons produced by mouse Ehrlich ascites tumor cells infected with Newcastle disease virus provides interferons…”
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Characterization of the intrinsic fibrinolytic properties of pro-urokinase through a study of plasmin-resistant mutant forms produced by site-specific mutagenesis of lysine(158)
Published in The Journal of clinical investigation (01-12-1988)“…Two plasmin-resistant mutant forms of pro-urokinase (pro-UK) constructed by site-directed mutagenesis of Lys158 to Val158 and Met158 were used to evaluate the…”
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Enzyme Membrane Immunoassay (EMIA)
Published in Bio/technology (New York, N.Y. 1983) (01-01-1984)Get full text
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Mouse Interferons: Amino Terminal Amino Acid Sequences of Various Species
Published in Science (American Association for the Advancement of Science) (01-02-1980)“…Mouse interferons of three size classes (A, 35,000 to 40,000 daltons; B, 26,000 to 33,000 daltons; and C, 20,000 daltons) were purified from Ehrlich ascites…”
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Rapid, homogeneous phase, liposome-based assays for total complement activity
Published in Complement (Basel) (1984)“…A simple, rapid assay for determining total complement activity has been developed. Complement activity is quantitated spectrophotometrically by measuring the…”
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Studies with pure mouse Ehrlich ascites tumor interferons alpha and beta: patterns of induction of (2'-5') (A)n synthetase and of a double-stranded RNA-dependent protein kinase in mouse cells and human cells
Published in Journal of interferon research (01-02-1981)“…The N-terminal sequences of mouse Ehrlich ascites tumor cell IFN beta (35,000-40,000 daltons) and IFN alpha (20,000 daltons) differ in 18 out of 20 positions…”
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Accumulation of an mRNA and protein in interferon-treated Ehrlich ascites tumour cells
Published in Nature (London) (07-06-1979)Get full text
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Characterization of the intrinsic fibrinolytic properties of pro-urokinase through a study of plasmin-resistant mutant forms produced by site-specific mutagenesis of lysine super(158)
Published in The Journal of clinical investigation (01-01-1988)“…Two plasmin-resistant mutant forms of pro-urokinase (pro-UK) constructed by site-directed mutagenesis of Lys super(158) to Val super(158) and Met super(158)…”
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