Search Results - "Broadwater, John A."

Discovery and optimization of oxadiazole-based FLAP inhibitors by Bartolozzi, Alessandra, Abeywardane, Asitha, Bosanac, Todd, Broadwater, John A., Chen, Zhidong, Hutzler, J. Matthew, Huber, John D., Nemoto, Peter, Olague, Alan, Riether, Doris, Simpson, Tom, Takahashi, Hidenori, Wu, Lifen, Zhang, Yunlong, Zindell, Renee M.

“…[Display omitted] Structure activity relationship (SAR) investigation of an oxadiazole based series led to the discovery of several potent FLAP inhibitors…”
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Desaturation and hydroxylation. Residues 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity by Broadwater, John A, Whittle, Edward, Shanklin, John

“…Exchanging the identity of amino acids at four key locations within the Arabidopsis thaliana oleate desaturase (FAD2) and the Lesquerella fendleri…”
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Mössbauer Studies of the Formation and Reactivity of a Quasi-Stable Peroxo Intermediate of Stearoyl-Acyl Carrier Protein Δ9-Desaturase by Broadwater, John A, Achim, Catalina, Münck, Eckard, Fox, Brian G

“…Stearoyl-ACP Δ9-desaturase (Δ9D) is a diiron enzyme that catalyzes 18:0-ACP desaturation. Each subunit of homodimeric resting Δ9D contains a diferric cluster,…”
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Circular Dichroism and Magnetic Circular Dichroism Studies of the Reduced Binuclear Non-Heme Iron Site of Stearoyl-ACP Δ9-Desaturase:  Substrate Binding and Comparison to Ribonucleotide Reductase by Yang, Yi-Shan, Broadwater, John A, Pulver, Sabine Coates, Fox, Brian G, Solomon, Edward I

“…Stearoyl-acyl carrier protein (stearoyl-ACP) Δ9-desaturase (Δ9D) catalyzes the insertion of a cis double bond between the 9 and 10 positions of the…”
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Peroxodiferric Intermediate of Stearoyl-Acyl Carrier Protein Δ9 Desaturase:  Oxidase Reactivity during Single Turnover and Implications for the Mechanism of Desaturation by Broadwater, John A, Ai, Jingyuan, Loehr, Thomas M, Sanders-Loehr, Fox, Brian G

“…Combined optical and resonance Raman studies have revealed the formation of an O2-adduct upon exposure of 4e- chemically reduced stearoyl-acyl carrier protein…”
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Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase by Nesbitt, Natasha M., Baleanu-Gogonea, Camelia, Cicchillo, Robert M., Goodson, Kathy, Iwig, David F., Broadwater, John A., Haas, Jeffrey A., Fox, Brian G., Booker, Squire J.

“…Lipoic acid is a sulfur-containing 8-carbon fatty acid that functions as a central cofactor in multienzyme complexes that are involved in the oxidative…”
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A structural role for arginine in proteins: Multiple hydrogen bonds to backbone carbonyl oxygens by Borders, C.L., Broadwater, John A., Bekeny, Paula A., Salmon, Johanna E., Lee, Ann S., Eldridge, Aimee M., Pett, Virginia B.

“…We propose that arginine side chains often play a previously unappreciated general structural role in the maintenance of tertiary structure in proteins,…”
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Desaturation and Hydroxylation by Broadwater, John A., Whittle, Edward, Shanklin, John

“…Exchanging the identity of amino acids at four key locations within the Arabidopsis thaliana oleate desaturase (FAD2) and the Lesquerella…”
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Mössbauer studies of the formation and reactivity of a quasi-stable peroxo intermediate of stearoyl-acyl carrier protein Delta 9-desaturase by Broadwater, J A, Achim, C, Münck, E, Fox, B G

“…Stearoyl-ACP Delta(9)-desaturase (Delta 9D) is a diiron enzyme that catalyzes 18:0-ACP desaturation. Each subunit of homodimeric resting Delta 9D contains a…”
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Spinach holo-acyl carrier protein: overproduction and phosphopantetheinylation in Escherichia coli BL21(DE3), in vitro acylation, and enzymatic desaturation of histidine-tagged isoform I by Broadwater, J A, Fox, B G

“…Spinach ACP isoform I was overexpressed in Escherichia coli BL21(DE3) using a gene synthesized from codons associated with high-level expression in E. coli…”
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Spinach Holo-Acyl Carrier Protein: Overproduction and Phosphopantetheinylation inEscherichia coliBL21(DE3),in VitroAcylation, and Enzymatic Desaturation of Histidine-Tagged Isoform I by Broadwater, John A., Fox, Brian G.

“…Spinach ACP isoform I was overexpressed inEscherichia coliBL21(DE3) using a gene synthesized from codons associated with high-level expression inE. coli.The…”
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Desaturation of trans-octadecenoyl-acyl carrier protein by stearoyl-acyl carrier protein Δ 9 desaturase by Broadwater, John A., Laundre, Bryan J., Fox, Brian G.

“…Positional isomers of mono-unsaturated 18:1-ACP have been used as substrates for stearoyl-acyl carrier protein Δ 9 desaturase to test whether a C–H bond…”
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Desaturation of trans-octadecenoyl-acyl carrier protein by stearoyl-acyl carrier protein Δ9 desaturase by Broadwater, John A., Laundre, Bryan J., Fox, Brian G.

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Lactose Fed-Batch Overexpression of Recombinant Metalloproteins in Escherichia coli BL21(DE3): Process Control Yielding High Levels of Metal-Incorporated, Soluble Protein by Hoffman, B.J., Broadwater, J.A., Johnson, P., Harper, J., Fox, B.G., Kenealy, W.R.

“…A method for producing recombinant proteins in pilot scale fermentation equipment using a glucose fed-batch initial growth, followed by a midlog phase feeding…”
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Circular Dichroism and Magnetic Circular Dichroism Studies of the Reduced Binuclear Non-Heme Iron Site of Stearoyl-ACP Δ 9 -Desaturase:  Substrate Binding and Comparison to Ribonucleotide Reductase by Yang, Yi-Shan, Broadwater, John A., Pulver, Sabine Coates, Fox, Brian G., Solomon, Edward I.

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EXAFS and Mössbauer characterization of the Diiron(III) site in stearoyl-acyl carrier protein Δ9– desaturase by Shu, Lijin, Broadwater, John A., Achim, Catalina, Fox, Brian G., Münck, Eckard, Que Jr, L.

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Azide adducts of stearoyl-ACP desaturase: a model for μ-1,2 bridging by dioxygen in the binuclear iron active site by Ai, Jingyuan, Broadwater, John A., Loehr, Thomas M., Sanders-Loehr, Joann, Fox, B. G.

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Peroxodiferric intermediate of stearoyl-acyl carrier protein delta 9 desaturase: oxidase reactivity during single turnover and implications for the mechanism of desaturation by Broadwater, J A, Ai, J, Loehr, T M, Sanders-Loehr, J, Fox, B G

“…Combined optical and resonance Raman studies have revealed the formation of an O2-adduct upon exposure of 4e- chemically reduced stearoyl-acyl carrier protein…”
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The fundamental, versatile role of diiron enzymes in lipid metabolism by Broadwater, John A., Haas, Jeffrey A., Fox, Brian G.

“…Diiron enzymes catalyze many essential O2‐dependent reactions required for eukaryotic and bacterial metabolism. Both membrane bound and soluble classes are now…”
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