Search Results - "Brlansky, R.H."

Evaluation of four phloem-specific promoters in vegetative tissues of transgenic citrus plants by Dutt, M, Ananthakrishnan, G, Jaromin, M.K, Brlansky, R.H, Grosser, J.W

“…‘Mexican’ lime (Citrus aurantifolia Swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolC promoter…”
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Production of monoclonal antibodies for detection of Citrus leprosis virus C in enzyme-linked immuno-assays and immunocapture reverse transcription-polymerase chain reaction by Choudhary, Nandlal, Roy, Avijit, Govindarajulu, A, Nakhla, M.K., Levy, L., Brlansky, R.H.

“…•MAbs were produced to the purified expressed coat protein of Citrus leprosis virus C.•MAbs C11, E10, F9 and G10 were selected to evaluate for the detection of…”
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A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees by Roy, Avijit, Fayad, Amer, Barthe, G., Brlansky, R.H.

“…A multiplex polymerase chain reaction (mPCR) assay was developed to detect six RNA and one DNA citrus virus: Citrus leaf rugose virus (CLRV), Citrus psorosis…”
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Immunodiagnosis of Citrus leprosis virus C using a polyclonal antibody to an expressed putative coat protein by Choudhary, Nandlal, Roy, Avijit, Guillermo, Leon M., Picton, D.D., Wei, G., Nakhla, M.K., Levy, L., Brlansky, R.H.

“…•The p29 gene encoding for putative coat protein of Citrus leprosis virus C (CiLV-C) were optimized for the expression in Escherichia coli.•The optimized p29…”
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Transmission Parameters for Candidatus Liberibacter asiaticus by Asian Citrus Psyllid (Hemiptera: Psyllidae) by Pelz-Stelinski, K. S, Brlansky, R. H, Ebert, T. A, Rogers, M. E

“…The purpose of this investigation was to evaluate acquisition and inoculation (together, transmission) efficiency of Candidatus Liberibacter asiaticus (Las),…”
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Inefficient Transmission of Citrus Tristeza Virus from Grapefruit by Single Brown Citrus Aphids by Lin, Youjian, Brlansky, R.H, Powell, Charles A

“…Six severe and six mild Florida isolates of citrus tristeza virus (CTV) were used to evaluate the transmission efficiency of the virus from grapefruit…”
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Genome analysis of an orange stem pitting citrus tristeza virus isolate reveals a novel recombinant genotype by Roy, Avijit, Brlansky, R.H.

“…An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular…”
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Changes in carbohydrate metabolism in Citrus sinensis infected with ‘Candidatus Liberibacter asiaticus by Fan, J, Chen, C, Brlansky, R.H, Gmitter, F.G. Jr, Li, Z.‐G

“…Changes in carbohydrate metabolism in sweet orange (Citrus sinensis) infected with ‘Candidatus Liberibacter asiaticus', a purported cause of citrus…”
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Population Dynamics of a Florida Citrus tristeza virus Isolate and Aphid-Transmitted Subisolates: Identification of Three Genotypic Groups and Recombinants After Aphid Transmission by Roy, Avijit, Brlansky, R.H

“…Tristeza is an important citrus disease affecting the viability and productivity of citrus worldwide. The causal agent, Citrus tristeza virus (CTV), usually…”
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Production of mono- and polyclonal antibodies to Citrus leprosis virus C2 and their application in triple antibody sandwich ELISA and immunocapture RT-PCR diagnostic assays by Choudhary, Nandlal, Roy, Avijit, Leon, M.G., Wei, G., Nakhla, M.K., Levy, L., Brlansky, R.H.

“…•Putative coat protein gene p29 of Citrus leprosis virus C2 (CiLV-C2) was amplified.•p29 gene was cloned and putative coat protein was expressed in E…”
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Development and Application of a Multiplex Reverse-Transcription Polymerase Chain Reaction Assay for Screening a Global Collection of Citrus tristeza virus Isolates by Roy, Avijit, Ananthakrishnan, G, Hartung, John S, Brlansky, R.H

“…The emerging diversity of Citrus tristeza virus (CTV) genotypes has complicated detection and diagnostic measures and prompted the search for new…”
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Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays by Choudhary, Nandlal, Wei, G., Govindarajulu, A., Roy, Avijit, Li, Wenbin, Picton, Deric. D., Nakhla, M.K., Levy, L., Brlansky, R.H.

“…•Specific primers and probe were designed targeting movement protein gene of CiLV-C.•Primers and probe set were amplifying a target specific 73bp fragment from…”
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Characterization of the mixture of genotypes of a Citrus tristeza virus isolate by reverse transcription-quantitative real-time PCR by Ananthakrishnan, G., Venkataprasanna, T., Roy, Avijit, Brlansky, R.H.

“…A multiplex real-time PCR assay was developed to detect and quantify the Citrus tristeza virus (CTV) genotypic mixture present in infected plants. CTV isolate…”
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Assessment of sequence diversity in the 5′-terminal region of Citrus tristeza virus from India by Roy, Avijit, Manjunath, K.L., Brlansky, R.H.

“…Twenty-one Citrus tristeza virus (CTV) isolates from India were characterized, using genotype-specific multiple molecular markers (MMM) from the 54′-terminal…”
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Genotype classification and molecular evidence for the presence of mixed infections in Indian Citrus tristeza virus isolates by Roy, A, Brlansky, R.H

“…Citrus tristeza virus (CTV) is usually present in field trees as a mixture or complex of isolates. Different biological types of CTV exist in India and this…”
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Mating and pseudothecial development in Mycosphaerella citri, the cause of citrus greasy spot by Mondal, S.N, Howd, D.S, Brlansky, R.H, Timmer, L.W

“…Greasy spot, caused by Mycosphaerella citri, is a serious disease of citrus in the Caribbean basin. M. citri is a loculoascomycete and produces pseudothecia in…”
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Citrus variegated chlorosis bacterium: axenic culture, pathogenicity, and serological relationships with other strains of Xylella fastidiosa by Hartung, J.S. (USDA, ARS, Beltsville Agriculture Research Center-West, Beltsville, MD.), Beretta, J, Brlansky, R.H, Spisso, J, Lee, R.F

“…A xylem-limited bacterium serologically related to strains of Xylella fastidiosa has been associated previously with citrus variegated chlorosis a new and…”
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Infection of flower and vegetative tissues of citrus by Colletotrichum acutatum and C. gloeosporioides by Zulfiqar, M. (University of Florida, Lake Alfred, FL.), Brlansky, R.H, Timmer, L.W

“…Colletotrichum acutatum causes postbloom fruit drop of citrus, whereas C. gloeosporioides causes postharvest anthracnose on fruit but does not affect healthy…”
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Use of immunogold labelling with scanning electron microscopy to identify phytopathogenic bacteria on leaf surfaces by Davis, C.L. (University of Florida, Lake Alfred, FL), Brlansky, R.H

“…Scanning electron microscopy coupled with high-resolution back-scattered electron imaging was used to detect gold-labelled specific immunoglobulins attached to…”
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Colonization of the sharpshooter vectors, Oncometopia nigricans and Homalodisca coagulata, by xylem-limited bacteria [Carriers of xylem-limited bacterial disease] by Brlansky, R. H.

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