Streptomyces thermocerradoensis I3 secretes a novel bifunctional xylanase/endoglucanase under solid‐state fermentation

Streptomyces thermocerradoensis I3 secretes a novel bifunctional xylanase/endoglucanase under solid‐state fermentation

Lignocellulosic wastes can be potentially converted into several bioproducts such as glucose, xylo‐oligosaccharides, and bioethanol. Certain processes, such as enzymatic hydrolysis, are generally needed to convert biomass into bioproducts. The present study investigated the production of xylanases a...

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Bibliographic Details
Published in:Biotechnology progress Vol. 36; no. 2; pp. e2934 - n/a
Main Authors: Gama, Aline Rodrigues, Brito‐Cunha, Carolina Candida Q., Campos, Ivan T. N., Souza, Guilherme Rocha L., Carneiro, Lilian Carla, Bataus, Luiz Artur Mendes
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01-03-2020
Wiley Subscription Services, Inc
Subjects:
Bagasse
Biofuels
Cellulase
Endoglucanase
Enzymatic activity
Enzyme activity
Enzymes
Ethanol
Fermentation
Lignocellulose
lignocellulosic wastes
Oligosaccharides
pH effects
Saccharification
Streptomyces
Sugarcane
sugarcane bagasse
Wheat bran
Xylanase
xylo‐oligosaccharides
wheat bran
sugarcane bagasse
saccharification
xylo-oligosaccharides
lignocellulosic wastes
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Summary:Lignocellulosic wastes can be potentially converted into several bioproducts such as glucose, xylo‐oligosaccharides, and bioethanol. Certain processes, such as enzymatic hydrolysis, are generally needed to convert biomass into bioproducts. The present study investigated the production of xylanases and cellulases by Streptomyces thermocerradoensis I3 under solid‐state fermentation (SSF), using wheat bran as a low‐cost medium. The activities of xylanase and carboxymethyl cellulase (CMCase) were evaluated until 96 hr of incubation. The highest enzyme activity was observed after 72 hr of incubation. The crude enzyme extract was sequentially filtered, first using a 50 kDa filter, followed by a 30 kDa filter. Fraction 3 (F3) exhibited activities of both xylanase and CMCase. Xylanase and CMCase showed optimum activity at 70°C and pH 6.0 and 55°C and pH 6.0, respectively. The zymogram analysis showed a single activity band with a molecular mass of approximately 17 kDa. These findings provide strong evidence that the enzyme is a bifunctional xylanase/endoglucanase. This enzyme improved the saccharification of sugarcane bagasse by 1.76 times that of commercial cellulase. This enzyme has potential applications in various biotechnological procedures.
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ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.2934