Simultaneous multiple injection in monosegmented flow analysis

Simultaneous multiple injection in monosegmented flow analysis

A flow approach for simultaneous multiple injection (SMI) in monosegmented flow analysis (MSFA) is described. In this approach, the sample and the reagent (or other solution, such as a diluting fluid) are simultaneously injected into the reaction coil of a monosegmented flow analyser. The monosegmen...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta Vol. 371; no. 2; pp. 317 - 324
Main Authors: Brito, Vanessa O., Raimundo, Ivo M.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 05-10-1998
Elsevier
Subjects:
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Monosegmented flow analysis
Oses
Other biological molecules
Schlieren effect
Simultaneous multiple injection
Monosegmented flow analysis
Schlieren effect
Simultaneous multiple injection
Human
Biological fluid
Flow(fluid)
Liquid liquid extraction
Analytical method
Serum
Carbohydrate
Glucose
Quantitative analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A flow approach for simultaneous multiple injection (SMI) in monosegmented flow analysis (MSFA) is described. In this approach, the sample and the reagent (or other solution, such as a diluting fluid) are simultaneously injected into the reaction coil of a monosegmented flow analyser. The monosegment is homogenised while it is carried towards the detector. The sample dilution procedure is not based on a gradient concentration pattern and dilution factors up to 150 were obtained by using hydrodynamic sampling. The system was applied to perform sample dilution in the determination of glucose in blood plasma. The results showed good correlation with those obtained by the Clinical Hospital of UNICAMP. The SMI approach was also applied to add reagent to the sample in nitrite determination in natural water, providing a methodology that has a sampling frequency of 72 h −1, a relative standard deviation of <2%, at 60 μg l −1 N-NO − 2, a linear response range up to 260 μg l −1 and a 3 s limit of detection of 5 μg l −1 N-NO − 2. Thus, the sensitivity is close to that of the manual reference method. Recovery tests carried out with sea water samples also showed that MSFA overcomes the Schlieren effect, without needing any special procedure.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(98)00321-3