Improvement in menthol extraction of fresh leaves of Mentha arvensis by the application of multi-enzymatic extract of Aspergillus niger

The objective of this study was to optimize the enzymatic pretreatment process of extracting menthol, a major component of the essential oil stored in the trichomes of fresh leaves of Mentha arvensis, by the action of the crude enzymatic extract previously produced by the Aspergillus niger fungus cu...

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Bibliographic Details
Published in:	Chemical engineering communications Vol. 206; no. 3; pp. 387 - 397
Main Authors:	Reis, Nadabe S., Brito, Aila R., Pacheco, Clissiane S. V., Costa, Larissa C. B., Gross, Eduardo, Santos, Tatielle P., Costa, Analu R., Silva, Erik G. P., Oliveira, Rosilene A., Aguiar-Oliveira, Elizama, Oliveira, Julieta R., Franco, Marcelo
Format:	Journal Article
Language:	English
Published:	Philadelphia Taylor & Francis 04-03-2019 Taylor & Francis Ltd
Subjects:	Cactus pear Design of experiments Enzymatic pretreatment Gas chromatography Glandular trichomes Hydrodistillation Menthol Organic chemistry Pretreatment Scanning electron microscopy Solid state fermentation
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Summary:	The objective of this study was to optimize the enzymatic pretreatment process of extracting menthol, a major component of the essential oil stored in the trichomes of fresh leaves of Mentha arvensis, by the action of the crude enzymatic extract previously produced by the Aspergillus niger fungus cultivated in cactus pear. Under the experimental conditions of 42 °C, 141 min and 58 mL of enzyme in 442 mL of water, there was an increase of 186.63% in the hydrodistillation yield, which represents an acceptable positive deviation of 12.54% compared to the predicted value of the model obtained by experimental design. Trichome rupture can be confirmed by scanning electron microscopy (SEM) and the chemical structure of the obtained menthol was not modified according to gas chromatography and infrared spectroscopy. The enzymatic pretreatment applied before the hydrodistillation step was successful and proves the potential of the association of biotechnological techniques with a physicochemical process.
ISSN:	0098-6445 1563-5201
DOI:	10.1080/00986445.2018.1494580