Human Langerhans Cells Are More Efficient Than CD14−CD1c+ Dermal Dendritic Cells at Priming Naive CD4+ T Cells

Few data are available regarding the role of human skin dendritic cells (DCs) in driving T-cell responses. In this study we analyzed the relative capacity of Langerhans cells (LCs) and dermal CD14−CD1c+ DCs (DDCs) to trigger naive CD4+ T-cell proliferation and differentiation. DC subsets were purifi...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of investigative dermatology Vol. 130; no. 5; pp. 1345 - 1354
Main Authors:	Furio, Laetitia, Briotet, Isabelle, Journeaux, Alexandra, Billard, Hermine, Péguet-Navarro, Josette
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-05-2010 Nature Publishing Group Elsevier Limited
Subjects:	Antigens, CD - metabolism Antigens, CD1 - metabolism B7-H1 Antigen Biological and medical sciences CD40 Ligand - pharmacology Cell Communication - immunology Cell Differentiation - immunology Cell Division - immunology Cell Movement - immunology Dermatology Down-Regulation - immunology Glycoproteins - metabolism Humans Immunophenotyping Interferon-gamma - pharmacology Isoantigens - immunology Langerhans Cells - cytology Langerhans Cells - immunology Langerhans Cells - metabolism Lipopolysaccharide Receptors - metabolism Lymphocyte Activation - drug effects Lymphocyte Activation - immunology Medical sciences Th1 Cells - cytology Th1 Cells - immunology Th2 Cells - cytology Th2 Cells - immunology Human Dendritic cell Langerhans cell Derm Dermatology T-Lymphocyte
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Few data are available regarding the role of human skin dendritic cells (DCs) in driving T-cell responses. In this study we analyzed the relative capacity of Langerhans cells (LCs) and dermal CD14−CD1c+ DCs (DDCs) to trigger naive CD4+ T-cell proliferation and differentiation. DC subsets were purified after a 2-day migration from epidermis and dermis of the same skin sample. Migratory LCs showed far more activated phenotype than CD1c+DDCs and distinct expression of new molecules of the B7 family; when compared with LCs, CD1c+DDCs showed higher PD-L1 and lower inducible co-stimulator ligand (ICOS-L) expression. As expected, CD1c+DDCs showed lower allostimulatory property than LCs, a process that was partly reversed by anti-PD-L1 mAb. LCs were significantly more efficient than CD1c+DDCs at inducing allogeneic naive CD4+ T cells to secrete both T helper cell 1 (Th1; IFN-γ and tumor necrosis factor-α ) and Th2 (IL-4 and IL-5) cytokines. Moreover, anti-PD-L1 mAb increased the production of IFN-γ by both LC- and CD1c+DDC-stimulated T cells. Globally, these results argue for a preponderant role of human LCs in inducing naive CD4+ T-cell priming. Low expression of co-stimulatory molecules together with high expression of PD-L1 might limit the efficiency of CD1c+DDCs at inducing naive CD4+ T-cell proliferation and secretion of cytokines.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-202X 1523-1747
DOI:	10.1038/jid.2009.424