Search Results - "Brinkmann, Ulrich"

Engineering therapeutic bispecific antibodies using CrossMab technology by Klein, Christian, Schaefer, Wolfgang, Regula, Joerg T., Dumontet, Charles, Brinkmann, Ulrich, Bacac, Marina, Umaña, Pablo

“…[Display omitted] •CrossMab technology enforce correct light chain in the Fab region of the bispecific antibody.•CrossMab technology is a versatile approach…”
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Format and geometries matter: Structure-based design defines the functionality of bispecific antibodies by Dickopf, Steffen, Georges, Guy J., Brinkmann, Ulrich

“…Multispecific antibodies can be generated in different formats. More than two decades of R&D in the field of bispecific antibody engineering revealed that the…”
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Quantitative fluorescence imaging determines the absolute number of locked nucleic acid oligonucleotides needed for suppression of target gene expression by Buntz, Annette, Killian, Tobias, Schmid, Daniela, Seul, Heike, Brinkmann, Ulrich, Ravn, Jacob, Lindholm, Marie, Knoetgen, Hendrik, Haucke, Volker, Mundigl, Olaf

“…Abstract Locked nucleic acid based antisense oligonucleotides (LNA-ASOs) can reach their intracellular RNA targets without delivery modules. Functional…”
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DPH1 and DPH2 variants that confer susceptibility to diphthamide deficiency syndrome in human cells and yeast models by Ütkür, Koray, Mayer, Klaus, Khan, Maliha, Manivannan, Thirishika, Schaffrath, Raffael, Brinkmann, Ulrich

“…ABSTRACT The autosomal-recessive diphthamide deficiency syndrome presents as intellectual disability with developmental abnormalities, seizures, craniofacial…”
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Format chain exchange (FORCE) for high-throughput generation of bispecific antibodies in combinatorial binder-format matrices by Dengl, Stefan, Mayer, Klaus, Bormann, Felix, Duerr, Harald, Hoffmann, Eike, Nussbaum, Bianca, Tischler, Michael, Wagner, Martina, Kuglstatter, Andreas, Leibrock, Lea, Buldun, Can, Georges, Guy, Brinkmann, Ulrich

“…Generation of bispecific antibodies (bsAbs) requires a combination of compatible binders in formats that support desired functionalities. Here, we report that…”
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Generation of binder-format-payload conjugate-matrices by antibody chain-exchange by Vasic, Vedran, Dickopf, Steffen, Spranger, Nadine, Rosenberger, Rose-Sophie, Fischer, Michaela, Mayer, Klaus, Larraillet, Vincent, Bates, Jack A., Maier, Verena, Sela, Tatjana, Nussbaum, Bianca, Duerr, Harald, Dengl, Stefan, Brinkmann, Ulrich

“…The generation of antibody-drug conjugates with optimal functionality depends on many parameters. These include binder epitope, antibody format, linker…”
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Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis by Zhang, Hongliang, Quintana, Julia, Ütkür, Koray, Adrian, Lorenz, Hawer, Harmen, Mayer, Klaus, Gong, Xiaodi, Castanedo, Leonardo, Schulten, Anna, Janina, Nadežda, Peters, Marcus, Wirtz, Markus, Brinkmann, Ulrich, Schaffrath, Raffael, Krämer, Ute

“…Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target…”
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Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast by Hawer, Harmen, Ütkür, Koray, Arend, Meike, Mayer, Klaus, Adrian, Lorenz, Brinkmann, Ulrich, Schaffrath, Raffael

“…In eukaryotes, the modification of an invariant histidine (His-699 in yeast) residue in translation elongation factor 2 (EF2) with diphthamide involves a…”
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Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies by Klein, Christian, Sustmann, Claudio, Thomas, Markus, Stubenrauch, Kay, Croasdale, Rebecca, Schanzer, Jürgen, Brinkmann, Ulrich, Kettenberger, Hubert, Regula, Jörg T., Schaefer, Wolfgang

“…The development of bispecific antibodies has attracted substantial interest, and many different formats have been described. Those specifically containing an…”
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DPH1 Gene Mutations Identify a Candidate SAM Pocket in Radical Enzyme Dph1•Dph2 for Diphthamide Synthesis on EF2 by Ütkür, Koray, Schmidt, Sarina, Mayer, Klaus, Klassen, Roland, Brinkmann, Ulrich, Schaffrath, Raffael

“…In eukaryotes, the Dph1•Dph2 dimer is a non-canonical radical SAM enzyme. Using iron-sulfur (FeS) clusters, it cleaves the cosubstrate S-adenosyl-methionine…”
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Functional Integrity of Radical SAM Enzyme Dph1•Dph2 Requires Non-Canonical Cofactor Motifs with Tandem Cysteines by Ütkür, Koray, Mayer, Klaus, Liu, Shihui, Brinkmann, Ulrich, Schaffrath, Raffael

“…The Dph1•Dph2 heterodimer from yeast is a radical SAM (RS) enzyme that generates the 3-amino-3-carboxy-propyl (ACP) precursor for diphthamide, a clinically…”
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Targeted chain-exchange-mediated reconstitution of a split type-I cytokine for conditional immunotherapy by Vasic, Vedran, Buldun, Can, Ritz, Manfred, Dickopf, Steffen, Georges, Guy J., Spick, Christian, Peuker, Alessa, Meier, Thomas, Mayer, Klaus, Brinkmann, Ulrich

“…Antibody-cytokine fusions targeted against tumor-associated antigens (TAAs) are promising cancer immunotherapy agents, with many such molecules currently…”
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Zirconium-89 Labeled Antibodies : A New Tool for Molecular Imaging in Cancer Patients by Perk, Lars, van de Watering, Floor C. J., Boerman, Otto C., Oyen, Wim J. G., Brinkmann, Ulrich, Rijpkema, Mark

“…Antibody based positron emission tomography (immuno-PET) imaging is of increasing importance to visualize and characterize tumor lesions. Additionally, it can…”
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Diphthamide affects selenoprotein expression: Diphthamide deficiency reduces selenocysteine incorporation, decreases selenite sensitivity and pre-disposes to oxidative stress by Mayer, Klaus, Mundigl, Olaf, Kettenberger, Hubert, Birzele, Fabian, Stahl, Sebastian, Pastan, Ira, Brinkmann, Ulrich

“…The diphthamide modification of translation elongation factor 2 is highly conserved in eukaryotes and archaebacteria. Nevertheless, cells lacking diphthamide…”
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Loss of diphthamide pre-activates NF-κB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor by Stahl, Sebastian, Seidl, Ana Rita da Silva Mateus, Ducret, Axel, van Geijtenbeek, Sabine Kux, Michel, Sven, Racek, Tomas, Birzele, Fabian, Haas, Alexander K., Rueger, Ruediger, Gerg, Michael, Niederfellner, Gerhard, Pastan, Ira, Brinkmann, Ulrich

“…The diphthamide on human eukaryotic translation elongation factor 2 (eEF2) is the target of ADP ribosylating diphtheria toxin (DT) andPseudomonasexotoxin A…”
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A Novel Glycoengineered Bispecific Antibody Format for Targeted Inhibition of Epidermal Growth Factor Receptor (EGFR) and Insulin-like Growth Factor Receptor Type I (IGF-1R) Demonstrating Unique Molecular Properties by Schanzer, Juergen M., Wartha, Katharina, Croasdale, Rebecca, Moser, Samuel, Künkele, Klaus-Peter, Ries, Carola, Scheuer, Werner, Duerr, Harald, Pompiati, Sandra, Pollman, Jan, Stracke, Jan, Lau, Wilma, Ries, Stefan, Brinkmann, Ulrich, Klein, Christian, Umana, Pablo

“…In the present study, we have developed a novel one-arm single chain Fab heterodimeric bispecific IgG (OAscFab-IgG) antibody format targeting the insulin-like…”
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Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2 by Arend, Meike, Ütkür, Koray, Hawer, Harmen, Mayer, Klaus, Ranjan, Namit, Adrian, Lorenz, Brinkmann, Ulrich, Schaffrath, Raffael

“…In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11…”
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A novel angiopoietin-2 selective fully human antibody with potent anti-tumoral and anti-angiogenic efficacy and superior side effect profile compared to Pan-Angiopoietin-1/-2 inhibitors by Thomas, Markus, Kienast, Yvonne, Scheuer, Werner, Bähner, Monika, Kaluza, Klaus, Gassner, Christian, Herting, Frank, Brinkmann, Ulrich, Seeber, Stefan, Kavlie, Anita, Welschof, Martin, Ries, Stefan, Weidner, K Michael, Regula, Jörg T, Klein, Christian

“…There is increasing experimental evidence for an important role of Angiopoietin-2 (Ang-2) in tumor angiogenesis and progression. In addition, Ang-2 is…”
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Characterization of a re-engineered, mesothelin-targeted Pseudomonas exotoxin fusion protein for lung cancer therapy by Bauss, Frieder, Lechmann, Martin, Krippendorff, Ben-Fillippo, Staack, Roland, Herting, Frank, Festag, Matthias, Imhof-Jung, Sabine, Hesse, Friederike, Pompiati, Marc, Kollmorgen, Gwendlyn, da Silva Mateus Seidl, Rita, Bossenmaier, Birgit, Lau, Wilma, Schantz, Christian, Stracke, Jan O., Brinkmann, Ulrich, Onda, Masanori, Pastan, Ira, Bosslet, Klaus, Niederfellner, Gerhard

“…Mesothelin overexpression in lung adenocarcinomas correlates with the presence of activating KRAS mutations and poor prognosis. Hence SS1P, a…”
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Bispecific digoxigenin-binding antibodies for targeted payload delivery by Metz, Silke, Haas, Alexander K, Daub, Karin, Croasdale, Rebecca, Stracke, Jan, Lau, Wilma, Georges, Guy, Josel, Hans-Peter, Dziadek, Sebastian, Hopfner, Karl-Peter, Lammens, Alfred, Scheuer, Werner, Hoffmann, Eike, Mundigl, Olaf, Brinkmann, Ulrich

“…Bispecific antibodies that bind cell-surface targets as well as digoxigenin (Dig) were generated for targeted payload delivery. Targeting moieties are IgGs…”
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