Search Results - "Briggs, John A.G."

Plasma Membrane Reshaping during Endocytosis Is Revealed by Time-Resolved Electron Tomography by Kukulski, Wanda, Schorb, Martin, Kaksonen, Marko, Briggs, John A.G.

“…Endocytosis, like many dynamic cellular processes, requires precise temporal and spatial orchestration of complex protein machinery to mediate membrane…”
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An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis by Skruzny, Michal, Desfosses, Ambroise, Prinz, Simone, Dodonova, Svetlana O., Gieras, Anna, Uetrecht, Charlotte, Jakobi, Arjen J., Abella, Marc, Hagen, Wim J.H., Schulz, Joachim, Meijers, Rob, Rybin, Vladimir, Briggs, John A.G., Sachse, Carsten, Kaksonen, Marko

“…Clathrin-mediated endocytosis, the main trafficking route from the plasma membrane to the cytoplasm, is critical to many fundamental cellular processes…”
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Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging by Hagen, Wim J.H., Wan, William, Briggs, John A.G.

“…Cryo-electron tomography (cryoET) allows 3D structural information to be obtained from cells and other biological samples in their close-to-native state. In…”
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Structures and distributions of SARS-CoV-2 spike proteins on intact virions by Ke, Zunlong, Oton, Joaquin, Qu, Kun, Cortese, Mirko, Zila, Vojtech, McKeane, Lesley, Nakane, Takanori, Zivanov, Jasenko, Neufeldt, Christopher J., Cerikan, Berati, Lu, John M., Peukes, Julia, Xiong, Xiaoli, Kräusslich, Hans-Georg, Scheres, Sjors H. W., Bartenschlager, Ralf, Briggs, John A. G.

“…Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by a lipid bilayer from which spike (S) protein trimers protrude 1 …”
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Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4 Å by Turoňová, Beata, Schur, Florian K.M., Wan, William, Briggs, John A.G.

“…Cryo-electron tomography (cryo-ET) allows cellular ultrastructures and macromolecular complexes to be imaged in three-dimensions in their native environments…”
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Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes by Rous, Brian A, Reaves, Barbara J, Ihrke, Gudrun, Briggs, John A G, Gray, Sally R, Stephens, David J, Banting, George, Luzio, J Paul

“…CD63 is a lysosomal membrane protein that belongs to the tetraspanin family. Its carboxyterminal cytoplasmic tail sequence contains the lysosomal targeting…”
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Vesicle coats: structure, function, and general principles of assembly by Faini, Marco, Beck, Rainer, Wieland, Felix T, Briggs, John A.G

“…The transport of proteins and lipids between distinct cellular compartments is conducted by coated vesicles. These vesicles are formed by the self-assembly of…”
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Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision by Kukulski, Wanda, Schorb, Martin, Welsch, Sonja, Picco, Andrea, Kaksonen, Marko, Briggs, John A.G

“…Correlative electron and fluorescence microscopy has the potential to elucidate the ultrastructural details of dynamic and rare cellular events, but has been…”
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Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy by Nikić, Ivana, Plass, Tilman, Schraidt, Oliver, Szymański, Jędrzej, Briggs, John A. G., Schultz, Carsten, Lemke, Edward A.

“…The growing demands of advanced fluorescence and super‐resolution microscopy benefit from the development of small and highly photostable fluorescent probes…”
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Endocytic sites mature by continuous bending and remodeling of the clathrin coat by Avinoam, Ori, Schorb, Martin, Beese, Carsten J., Briggs, John A. G., Kaksonen, Marko

“…During clathrin-mediated endocytosis (CME), plasma membrane regions are internalized to retrieve extracellular molecules and cell surface components. Whether…”
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Deciphering the Origin and Evolution of Hepatitis B Viruses by Means of a Family of Non-enveloped Fish Viruses by Lauber, Chris, Seitz, Stefan, Mattei, Simone, Suh, Alexander, Beck, Jürgen, Herstein, Jennifer, Börold, Jacob, Salzburger, Walter, Kaderali, Lars, Briggs, John A.G., Bartenschlager, Ralf

“…Hepatitis B viruses (HBVs), which are enveloped viruses with reverse-transcribed DNA genomes, constitute the family Hepadnaviridae. An outstanding feature of…”
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Molecular architecture of the inner ring scaffold of the human nuclear pore complex by Kosinski, Jan, Mosalaganti, Shyamal, von Appen, Alexander, Teimer, Roman, DiGuilio, Amanda L., Wan, William, Bui, Khanh Huy, Hagen, Wim J.H., Briggs, John A. G., Glavy, Joseph S., Hurt, Ed, Beck, Martin

“…Nuclear pore complexes (NPCs) are 110-megadalton assemblies that mediate nucleocytoplasmic transport. NPCs are built from multiple copies of ~30 different…”
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Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution by Schur, Florian K. M., Hagen, Wim J. H., Rumlová, Michaela, Ruml, Tomáš, Müller, Barbara, Kräusslich, Hans-Georg, Briggs, John A. G.

“…Cryo-electron tomography and subtomogram averaging methods reveal the structure of the capsid lattice within intact heterogeneous immature HIV-1 particles…”
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New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography by Schorb, Martin, Gaechter, Leander, Avinoam, Ori, Sieckmann, Frank, Clarke, Mairi, Bebeacua, Cecilia, Bykov, Yury S., Sonnen, Andreas F.-P., Lihl, Reinhard, Briggs, John A.G.

“…Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same…”
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High-throughput ultrastructure screening using electron microscopy and fluorescent barcoding by Bykov, Yury S, Cohen, Nir, Gabrielli, Natalia, Manenschijn, Hetty, Welsch, Sonja, Chlanda, Petr, Kukulski, Wanda, Patil, Kiran R, Schuldiner, Maya, Briggs, John A G

“…Genetic screens using high-throughput fluorescent microscopes have generated large datasets, contributing many cell biological insights. Such approaches cannot…”
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Cryo electron tomography of native HIV-1 budding sites by Carlson, Lars-Anders, de Marco, Alex, Oberwinkler, Heike, Habermann, Anja, Briggs, John A G, Kräusslich, Hans-Georg, Grünewald, Kay

“…The structure of immature and mature HIV-1 particles has been analyzed in detail by cryo electron microscopy, while no such studies have been reported for…”
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Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ by Zanetti, Giulia, Briggs, John A G, Grünewald, Kay, Sattentau, Quentin J, Fuller, Stephen D

“…The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for…”
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Structural organization of authentic, mature HIV-1 virions and cores by Briggs, John A. G., Wilk, Thomas, Welker, Reinhold, Kräusslich, Hans-Georg, Fuller, Stephen D.

“…Mature, infectious HIV‐1 particles contain a characteristic cone‐shaped core that encases the viral RNA and replication proteins. The architectures of mature…”
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Structural analysis of HIV-1 maturation using cryo-electron tomography by de Marco, Alex, Müller, Barbara, Glass, Bärbel, Riches, James D, Kräusslich, Hans-Georg, Briggs, John A G

“…HIV-1 buds form infected cells in an immature, non-infectious form. Maturation into an infectious virion requires proteolytic cleavage of the Gag polyprotein…”
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A saposin-lipoprotein nanoparticle system for membrane proteins by Frauenfeld, Jens, Löving, Robin, Armache, Jean-Paul, Sonnen, Andreas F-P, Guettou, Fatma, Moberg, Per, Zhu, Lin, Jegerschöld, Caroline, Flayhan, Ali, Briggs, John A G, Garoff, Henrik, Löw, Christian, Cheng, Yifan, Nordlund, Pär

“…A saposin protein–lipid nanoparticle system stabilizes diverse, fragile membrane proteins in a lipid environment for structural and functional studies. A…”
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