Dicentric Dose Estimates for Patients Undergoing Radiotherapy in the RTGene Study to Assess Blood Dosimetric Models and the New Bayesian Method for Gradient Exposure

The RTGene study was focused on the development and validation of new transcriptional biomarkers for prediction of individual radiotherapy patient responses to ionizing radiation. In parallel, for validation purposes, this study incorporated conventional biomarkers of radiation exposure, including t...

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Published in:	Radiation research Vol. 190; no. 6; pp. 596 - 604
Main Authors:	Moquet, Jayne, Higueras, Manuel, Donovan, Ellen, Boyle, Sue, Barnard, Stephen, Bricknell, Clare, Sun, Mingzhu, Gothard, Lone, O'Brien, Grainne, Cruz-Garcia, Lourdes, Badie, Christophe, Ainsbury, Elizabeth, Somaiah, Navita
Format:	Journal Article
Language:	English
Published:	United States The Radiation Research Society 01-12-2018 Radiation Research Society
Subjects:	Adult Aged Bayes Theorem Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Breast Neoplasms - blood Breast Neoplasms - pathology Breast Neoplasms - radiotherapy Chromosome Aberrations - radiation effects Chromosomes - genetics Chromosomes - radiation effects Dose-Response Relationship, Radiation Female Gastrointestinal Neoplasms - blood Gastrointestinal Neoplasms - pathology Gastrointestinal Neoplasms - radiotherapy Humans Lung Neoplasms - blood Lung Neoplasms - pathology Lung Neoplasms - radiotherapy Male Middle Aged Radiation Dosage Radiation, Ionizing Radiometry REGULAR ARTICLES Urogenital Neoplasms - blood Urogenital Neoplasms - pathology Urogenital Neoplasms - radiotherapy
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Summary:	The RTGene study was focused on the development and validation of new transcriptional biomarkers for prediction of individual radiotherapy patient responses to ionizing radiation. In parallel, for validation purposes, this study incorporated conventional biomarkers of radiation exposure, including the dicentric assay. Peripheral blood samples were taken with ethical approval and informed consent from a total of 20 patients undergoing external beam radiotherapy for breast, lung, gastrointestinal or genitourinary tumors. For the dicentric assay, two samples were taken from each patient: prior to radiotherapy and before the final fraction. Blood samples were set up using standard methods for the dicentric assay. All the baseline samples had dicentric frequencies consistent with the expected background for the normal population. For blood taken before the final fraction, all the samples displayed distributions of aberrations, which are indicative of partial-body exposures. Whole-body and partial-body cytogenetic doses were calculated with reference to a 250-kVp X-ray calibration curve and then compared to the dose to blood derived using two newly developed blood dosimetric models. Initial comparisons indicated that the relationship between these measures of dose appear very promising, with a correlation of 0.88 (P = 0.001). A new Bayesian zero-inflated Poisson finite mixture method was applied to the dicentric data, and partial-body dose estimates showed no significant difference (P > 0.999) from those calculated by the contaminated Poisson technique. The next step will be further development and validation in a larger patient group.
Bibliography:	Moquet, J., Higueras, M., Donovan, E., Boyle, S., Barnard, S., Bricknell, C., Sun, M., Gothard, L., O’Brien, G., Cruz-Garcia, L., Badie, C., Ainsbury, E. and Somaiah, N. Dicentric Dose Estimates for Patients Undergoing Radiotherapy in the RTGene Study to Assess Blood Dosimetric Models and the New Bayesian Method for Gradient Exposure. Radiat. Res. 190, 596–604 (2018).
ISSN:	0033-7587 1938-5404
DOI:	10.1667/RR15116.1