Hormonal Regulation of Androgen Receptor Messenger Ribonucleic Acid Expression in Human Tooth Pulp

Tooth pulp contains steroid receptors and therefore is likely to respond to steroids. Steroids and cytokines together can alter steroid receptor content in many tissues; thus, similar mechanisms may exist in tooth pulp. In this study, reverse-transcription/polymerase chain-reaction was used to scree...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of dental research Vol. 81; no. 5; pp. 360 - 365
Main Authors:	Dale, J.B., Sarich, S.L., Bretz, T.M., Hatton, J.F., Zachow, R.J.
Format:	Journal Article
Language:	English
Published:	United States SAGE Publications 01-05-2002 SAGE PUBLICATIONS, INC
Subjects:	Adolescent Adult Age Factors Analysis of Variance Androstenedione - physiology Cells, Cultured Dental Pulp - metabolism Dentistry Estradiol - physiology Estrogen Receptor beta Female Gene Expression Regulation Hormones - physiology Human Growth Hormone - physiology Humans Male Polymerase Chain Reaction Proto-Oncogene Proteins c-met - physiology Receptors, Androgen - biosynthesis Receptors, Estrogen - biosynthesis RNA, Messenger - analysis Sex Factors Statistics, Nonparametric tooth pulp androgen estrogen androgen receptor hepatocyte growth factor
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Tooth pulp contains steroid receptors and therefore is likely to respond to steroids. Steroids and cytokines together can alter steroid receptor content in many tissues; thus, similar mechanisms may exist in tooth pulp. In this study, reverse-transcription/polymerase chain-reaction was used to screen human pulp for the mRNAs encoding receptors for androgen (AR), estrogens (ERβ), and hepatocyte growh factor (HGF: c-Met). AR mRNA content was greater in male pulp vs. female pulp in all age groups. In both genders, AR mRNA content diminished with age. In pulp cell cultures, androstenedione, estradiol-17β, and HGF each stimulated AR mRNA accumulation. Testosterone inhibited, whereas 5α-dihydrotestosterone did not affect, AR mRNA content. ERβ was not hormonally altered in pulp cell cultures. By showing steroid- and cytokine-orchestrated regulation of AR mRNA in vitro, it is possible that age- and/or pathogen-dependent changes in available steroids and cytokines can affect any androgen-responsiveness of pulp.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-0345 1544-0591
DOI:	10.1177/154405910208100514